Methods for Diagnosing the Dumps, BLAD, HY and OH1 Hereditary Abnormalities in Cattle and Determining their Prevalence
Dyussekenova N. Nessipbayeva A. Buralkhiyev B. Khussainov D. Akimzhan N. Muslimova Z. Kalkayeva D. Turgumbekov A. Ussenbekov Y.
May-Jun, 2025Unique Scientific Publishers
International Journal of Veterinary Science
2025#14Issue 3595 - 604 pp.
The study aimed to improve the methods of diagnosis and study the prevalence of deficiency of uridine monophosphate synthase (DUMPS), bovine leukocyte adhesion deficiency (BLAD), hypotrichosis (HY), and achromatopsia (OH1) genetic defects in cattle bred in Kazakhstan. Genetic screening of 360 animals of the Kazakh white-headed, Hereford, and Angus breeds to detect the HY genetic defect was conducted using the tetra-primer amplification-refractory mutation system-PCR method developed by the authors. The BLAD incidence rate according to the results of double DNA certification in stud bulls from the Asyl Tulik JSC was 13.0% in the first study and 6.25% in the subsequent one. The advantage of the developed screening method is an increased rate and the exclusion of the use of restriction enzymes to identify wild and mutant allele types, which leads to a reduction in the cost of diagnostic testing. The study also determined the location of the point mutation c.751G>A in the hereditary eye disease OH1 in cattle. Based on this, a PCR-RFLP method for diagnosing this genetic defect in the Alatau breed was developed, and the prevalence of the OH1 mutation in the studied group of animals was 3.65%. A further strategy for eliminating detrimental mutations in breeding animals provides for genetic screening using modern molecular genetic diagnostic methods proposed in this paper.
Achromatopsia , Detrimental mutation elimination strategy , Hereditary abnormalities in cattle , Hypotrichosis
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Kazakh National Agrarian Research University, Almaty, Kazakhstan
Mukhtar Auezov South Kazakhstan University, Shymkent, Kazakhstan
Kazakh National Agrarian Research University
Mukhtar Auezov South Kazakhstan University
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