Nonspecific increase of αβTCR+ double-negative T cells in pediatric rheumatic diseases
Dossybayeva K. Zhubanova G. Mussayeva A. Mukusheva Z. Dildabayeva A. Nauryzbayeva G. Akhmaltdinova L. Orumbayeva U. Tanko M. Poddighe D.
December 2024Zhejiang University School of Medicine Childrens Hospital
World Journal of Pediatrics
2024#20Issue 121283 - 1292 pp.
Background: An increased number of double-negative T (DNT) cells expressing the αβ T cell receptor (αβ+DNT cells) is one of the diagnostic criteria for autoimmune lymphoproliferative syndrome (ALPS). Moreover, these cells are expanded in a widely used murine model for lupus. However, the homeostasis of αβ+DNT cells remains inadequately investigated in rheumatic disorders, especially in pediatric patients. Methods: In this cross-sectional, prospective, and observational study, children with rheumatic disorders and healthy controls were recruited to analyze the quantity and characteristics of circulating DNT cells using flow cytometry. Results: Overall, the two study groups did not differ in their total DNT cell pool in the bloodstream. However, the number of αβ+DNT cells was significantly higher in rheumatic children than that in the controls, whereas the γδ+DNT cells remained similar. This expansion in the circulating pool of αβ+DNT cells was comparable across different rheumatic diseases, all showing significant differences from the controls in this regard. Moreover, no significant correlation was found between αβ+DNT cell numbers and disease activity. Conclusions: These preliminary results indicate that circulating αβ+DNT cells are significantly expanded in children with rheumatic disorders; however, this finding appears to be a nonspecific (disease-unrelated) marker of autoimmunity. Further and larger studies are necessary to better investigate and define the role of DNT cells in pediatric rheumatic diseases.
DNT cells , Double-negative T cells , Juvenile idiopathic arthritis , Pediatric rheumatic disorders , Systemic lupus erythematosus
Text of the article Перейти на текст статьи
Department of Medicine, Nazarbayev University School of Medicine (NUSOM), Astana, 010000, Kazakhstan
Program of Pediatric Rheumatology, Clinical Academic Department of Pediatrics, University Medical Center, Astana, Kazakhstan
Clinical Academic Department of Laboratory Medicine, Pathology and Genetics, Republican Diagnostic Center, University Medical Center, Astana, Kazakhstan
Clinical Academic Department of Pediatrics, National Research Center for Maternal and Child Health (NRCMCH), University Medical Center(UMC), Astana, 010000, Kazakhstan
College of Health Sciences, VinUniversity, Gia Lam District, Hanoi, Viet Nam
Department of Medicine
Program of Pediatric Rheumatology
Clinical Academic Department of Laboratory Medicine
Clinical Academic Department of Pediatrics
College of Health Sciences
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026