Study of the Therapeutic Effect of Cytokine-Preconditioned Mesenchymal Stem Cells and Their Exosomes in a Mouse Model of Psoriasis
Dairov A. Issabekova A. Sarsenova M. Sekenova A. Shakhatbayev M. Alimbek S. Kudaibergen G. Nurkina A. Akhmetollayev I. Kang K.-S. Ogay V.
August 2025Multidisciplinary Digital Publishing Institute (MDPI)
Biology
2025#14Issue 8
Mesenchymal stem cells (MSCs) are a type of multipotent, non-hematopoietic cells of mesodermal origin. Due to their strong immunomodulatory, immunosuppressive, and regenerative potential, MSCs are used in cell therapy for inflammatory, immune-mediated, and degenerative diseases. Exosomes derived from MSCs have several advantages over MSC therapy, including non-immunogenicity, lack of infusion toxicity, ease of isolation, manipulation, and storage, cargo specificity, and the absence of tumor-forming potential and ethical concerns. We hypothesized that preconditioning human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) with the proinflammatory cytokines interleukin 17 (IL-17), IL-22, and tumor necrosis factor alpha (TNF-α), the increased levels of which are typical in psoriasis patients, can significantly increase the therapeutic efficacy of both hUCB-MSCs and their exosomes (hUCB-MSC-Exo). Our aim was to compare the therapeutic effects of hUCB-MSCs preconditioned with various combinations of proinflammatory cytokines and their hUCB-MSC-Exo, in an in vivo imiquimod-induced psoriasis-like skin inflammation model in mice. Our results showed a significant attenuation of psoriasis symptoms (erythema, scaling, and skin thickness) in mice treated with intact hUCB-MSCs, hUCB-MSCs preconditioned with IL-22 and TNF-α, and hUCB-MSC-Exo preconditioned with IL-17, IL-22 and TNF-α (MSC-Exo 3C). However, the most pronounced therapeutic effect was observed with MSC-Exo 3C treatment. In summary, we demonstrated that MSC-Exo 3C transplantation has therapeutic potential for treating psoriasis-like skin lesions.
cytokine , exosome , human umbilical cord blood mesenchymal stem cell , mesenchymal stem cell , psoriasis , skin inflammation
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Stem Cell Laboratory, National Center for Biotechnology, Astana, 010000, Kazakhstan
Department of General Biology and Genomics, L.N. Gumilyov Eurasian National University, Astana, 010008, Kazakhstan
Laboratory for Development of Molecular Diagnostic Approaches, National Center for Biotechnology, Astana, 010000, Kazakhstan
Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, 08826, South Korea
Stem Cell Laboratory
Department of General Biology and Genomics
Laboratory for Development of Molecular Diagnostic Approaches
Adult Stem Cell Research Center
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