Metabolic utilization of human osteoblast cell line hFOB 1.19 under normoxic and hypoxic conditions: A phenotypic microarray analysis
Cui Y.C. Qiu Y.S. Wu Q. Bu G. Peli A. Teh S.W. Ang K.P. Joseph N.M.S. Koh A.E.-H. Farhana A. Alzahrani B. Khan M.S.A. Samrot A.V. Mok P.L. Subbiah S.K.
May 2021SAGE Publications Inc.
Experimental Biology and Medicine
2021#246Issue 101177 - 1183 pp.
Osteoblasts play an important role in bone regeneration and repair. The hypoxia condition in bone occurs when bone undergoes fracture, and this will trigger a series of biochemical and mechanical changes to enable bone repair. Hence, it is interesting to observe the metabolites and metabolism changes when osteoblasts are exposed to hypoxic condition. This study has looked into the response of human osteoblast hFOB 1.19 under normoxic and hypoxic conditions by observing the cell growth and utilization of metabolites via Phenotype MicroArrays™ under these two different oxygen concentrations. The cell growth of hFOB 1.19 under hypoxic condition showed better growth compared to hFOB 1.19 under normal condition. In this study, osteoblast used glycolysis as the main pathway to produce energy as hFOB 1.19 in both hypoxic and normoxic conditions showed cell growth in well containing dextrin, glycogen, maltotriose, D-maltose, D-glucose-6-phospate, D-glucose, D-mannose, D-Turanose, D-fructose-6-phosphate, D-galactose, uridine, adenosine, inosine and α-keto-glutaric acid. In hypoxia, the cells have utilized additional metabolites such as α-D-glucose-1-phosphate and D-fructose, indicating possible activation of glycogen synthesis and glycogenolysis to metabolize α-D-glucose-1-phosphate. Meanwhile, during normoxia, D-L-α-glycerol phosphate was used, and this implies that the osteoblast may use glycerol-3-phosphate shuttle and oxidative phosphorylation to metabolize glycerol-3-phosphate.
glycerol-3-phosphate shuttle , glycolysis , hypoxic , Osteoblast , phenotype microarray
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Department of Rehabilitation Medicine, The First Affiliated Hospital of Xi’an JiaoTong University, Xi’An, 710061, China
Department of Orthopedic, Hospital of Xi’an JiaoTong University, Xi’An, 710061, China
Department of Medical Microbiology Parasitology, Faculty of Medicine Health Sciences, Universiti Putra Malaysia, Serdang, 43400,, Malaysia
Department of Biomedical Sciences, Faculty of Medicine Health Sciences, Universiti Putra Malaysia, Serdang, 43400, Malaysia
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka P.O Box 2014, Saudi Arabia
Department of Biomedical Sciences, School of Medicine, Nazarbayev University, Nur-Sultan, 010000, Kazakhstan
Department of Biomedical Sciences, Faculty of Medicine and Biomedical Sciences, MAHSA University, Selangor, 42810,, Malaysia
Genetics and Regenerative Medicine Research Centre, Faculty of Medicine Health Sciences, Universiti Putra Malaysia, Serdang, 43400, Malaysia
UPM‐MAKNA Cancer Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang, 43400, Malaysia
Department of Biotechnology, Bharath Institute of Higher Education and Research, Bharath University, Chennai, 600073, Tamil Nadu, India
Department of Rehabilitation Medicine
Department of Orthopedic
Department of Medical Microbiology Parasitology
Department of Biomedical Sciences
Department of Clinical Laboratory Sciences
Department of Biomedical Sciences
Department of Biomedical Sciences
Genetics and Regenerative Medicine Research Centre
UPM‐MAKNA Cancer Research Laboratory
Department of Biotechnology
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