Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
Coelho T. Sonnenberg-Riethmacher E. Gao Y. Mossotto E. Khojanazarov A. Griffin A. Mukanova S. Ashimkhanova A. Haggarty R. Borissenko A. Ashton J.J. Stafford I.S. Batra A. Afzal N.A. Stanton M.P. Vadgama B. Adrisova K. Beattie R.M. Williams A.P. Ennis S. Riethmacher D.
December 2021Nature Research
Scientific Reports
2021#11Issue 1
The precise role of periostin, an extra-cellular matrix protein, in inflammatory bowel disease (IBD) is unclear. Here, we investigated periostin in paediatric IBD including its relationship with disease activity, clinical outcomes, genomic variation and expression in the colonic tissue. Plasma periostin was analysed using ELISA in 144 paediatric patients and 38 controls. Plasma levels were assessed against validated disease activity indices in IBD and clinical outcomes. An immuno-fluorescence for periostin and detailed isoform-expression analysis in the colonic tissue was performed in 23 individuals. We integrated a whole-gene based burden metric ‘GenePy’ to assess the impact of variation in POSTN and 23 other genes functionally connected to periostin. We found that plasma periostin levels were significantly increased during remission compared to active Crohn’s disease. The immuno-fluorescence analysis demonstrated enhanced peri-cryptal ring patterns in patients compared to controls, present throughout inflamed, as well as macroscopically non-inflamed colonic tissue. Interestingly, the pattern of isoforms remained unchanged during bowel inflammation compared to healthy controls. In addition to its role during the inflammatory processes in IBD, periostin may have an additional prominent role in mucosal repair. Additional studies will be necessary to understand its role in the pathogenesis, repair and fibrosis in IBD.
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Human Genetics and Genomic Medicine, University of Southampton, Southampton, United Kingdom
Department of Biomedical Sciences, Nazarbayev University School of Medicine, Nur-Sultan, Kazakhstan
Department of Paediatric Gastroenterology, University Hospital Southampton, Southampton, United Kingdom
Academic Unit of Human Development and Health, University of Southampton, Southampton, United Kingdom
Cancer Sciences Division, Faculty of Medicine, University Hospital Southampton, Southampton, United Kingdom
Southampton Biomedical Research Centre, NIHR, University Hospital Southampton, Southampton, United Kingdom
Department of Paediatric Surgery, University Hospital Southampton, Southampton, United Kingdom
Department of Pathology, University Hospital Southampton, Southampton, United Kingdom
National Research Center for Maternal and Child Health, Nur-Sultan, Kazakhstan
Organ Transplantation Center, The First Affiliated Hospital, Sun Yatsen University, Guangzhou, China
Human Genetics and Genomic Medicine
Department of Biomedical Sciences
Department of Paediatric Gastroenterology
Academic Unit of Human Development and Health
Cancer Sciences Division
Southampton Biomedical Research Centre
Department of Paediatric Surgery
Department of Pathology
National Research Center for Maternal and Child Health
Organ Transplantation Center
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