Gene editing to facilitate hybrid crop production
Chen G. Zhou Y. Kishchenko O. Stepanenko A. Jatayev S. Zhang D. Borisjuk N.
1 January 2021Elsevier Inc.
Biotechnology Advances
2021#46
Capturing heterosis (hybrid vigor) is a promising way to increase productivity in many crops; hybrid crops often have superior yields, disease resistance, and stress tolerance compared with their parental inbred lines. The full utilization of heterosis faces a number of technical problems related to the specifics of crop reproductive biology, such as difficulties with generating and maintaining male-sterile lines and the low efficiency of natural cross-pollination for some genetic combinations. Innovative technologies, such as development of artificial in vitro systems for hybrid production and apomixis-based systems for maintenance of the resulting heterotic progeny, may substantially facilitate the production of hybrids. Genome editing using specifically targeted nucleases, such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (CRISPR/Cas9) systems, which recognize targets by RNA:DNA complementarity, has recently become an integral part of research and development in life science. In this review, we summarize the progress of genome editing technologies for facilitating the generation of mutant male sterile lines, applications of haploids for hybrid production, and the use of apomixis for the clonal propagation of elite hybrid lines.
Apomixis , CRISPR/Cas9 , Crop hybrids , Gene editing , Haploids , Heterosis , Male fertility
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Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, School of Life Sciences, Huaiyin Normal University, Huaian, China
Jiangsu Collaborative Innovation Centre of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huaian, China
Institute of Cell Biology & Genetic Engineering, National Academy of Science of Ukraine, Kyiv, Ukraine
Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University, Nur-Sultan, Kazakhstan
Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, Australia
Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake
Jiangsu Collaborative Innovation Centre of Regional Modern Agriculture & Environmental Protection
Institute of Cell Biology & Genetic Engineering
Faculty of Agronomy
Joint International Research Laboratory of Metabolic & Developmental Sciences
School of Agriculture
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