Diagnostic Usefulness of Combined Periplasmic Proteins in Bovine Brucellosis

Bulashev A. Mukhlis S. Manat Y. Mukhambetova A. Berdikulov M. Karibayev T. Bilyalov Y. Kaukabayeva G. Eskendirova S.
Mar-Apr 2025 Unique Scientific Publishers

International Journal of Veterinary Science
2025 #14 Issue 2 316 - 324 pp.

The low efficiency of brucellosis eradication efforts is primarily due to the low performance of conventional serological tests. This is because both traditional and modern tests use the whole cell of the pathogen or its lipopolysaccharides as an antigen, which can lead not only to false-positive results due to antibodies against related bacteria but also to a decrease in the sensitivity of the analysis. The world of veterinary sciences has been actively involved in the search for an immuno-reactive non-polysaccharide antigen specific for Brucella spp. that may hold the key to a promising diagnostic test. This study evaluated the serological potential of Brucella combined recombinant periplasmic proteins BP26 and superoxide dismutase (SOD) as antigens using indirect enzyme-linked immunosorbent assay ELISA (i-ELISA). The combined antigen was specific for Brucella spp., was not recognized by antisera against closely related bacteria, including Yersinia enterocolitica O:9 and did not bind to antibodies from negative bovine sera. Blood sera from brucellosis-positive cattle (n=1541), healthy cows (n=608) and brucellosis-free calves (n=46) were used. The efficacy of the combined antigen-based i-ELISA was evaluated by comparing its diagnostic performance with those of the rose Bengal plate test (RBPT), complement fixation test (CFT) and tube agglutination test (TAT). The i-ELISA/BP26+SOD demonstrated the following: sensitivity, 91.6%; specificity, 98.8%; accuracy, 93.6%; positive likelihood ratio, 76.3%; negative likelihood ratio, 0.08%; positive predictive value, 99.5%; and negative predictive value, 82.3%. The kappa coefficient between the serological tests was 0.85, indicating excellent agreement. i-ELISA/BP26 + SOD may be recommended as a supplementary test for brucellosis diagnosis.

Brucellosis , Cattle , Conventional serological tests , Diagnosis , Enzyme-linked immunosorbent assay , Recombinant periplasmic proteins

Text of the article Перейти на текст статьи

Department of Microbiology and Biotechnology, Faculty of Veterinary Medicine and Livestock Technology, S. Seifullin Kazakh Agrotechnical Research University, Astana, 010011, Kazakhstan
Stem Cell Laboratory, National Center for Biotechnology, Astana, 010000, Kazakhstan
Infectious Disease Diagnostic Laboratory, National Reference Center for Veterinary Medicine, Astana, 010000, Kazakhstan
Department of Veterinary Science, Shakarim University of Semey, Semey, 070000, Kazakhstan

Department of Microbiology and Biotechnology
Stem Cell Laboratory
Infectious Disease Diagnostic Laboratory
Department of Veterinary Science

10 лет помогаем публиковать статьи Международный издатель

Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026