Optimization of culture medium for enhanced protease biosynthesis in streptomyces globisporus
Blieva R.K. Mustafin K.G. Akhmetsadykov N.N. Suleimenova Z.B. Kalieva A.K. Narmuratova Z.B. Saduyeva Z.K. Zhakipbekova A.S. Tapenbayeva I.E.
2021Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma
Rasayan Journal of Chemistry
2021#14Issue 1270 - 275 pp.
In present study screening of strain strongly producing protease enzyme was conducted. Streptomyces globisporus 1/68 was found to possess maximum protein degrading capacity. The effect of carbon sources, inorganic and organic nitrogen sources on protease production by Streptomyces globisporus 1/68 was evaluated. The optimal carbon and nitrogen sources in the nutrient medium were selected. Maximal protease production was shown in medium with galactose as a carbon source (1.2 U/ml). Among inorganic nitrogen sources tested ammonium dihydrogen phosphate (NH4H2PO4) supported moderate growth and protease production in Streptomyces globisporus 1/68 with enzymatic activity of 5.8 U/ml. The addition of organic nitrogen sources did not affect the protease biosynthesis in tested culture. Besides, amino acids supplemented in medium with selected nitrogen inorganic source had no significant effect on protease biosynthesis in by Streptomyces globisporus 1/68.
Carbon Sources , Growth Conditions , Microbial Enzymes , Nitrogen Sources , Protease , Streptomyces globisporus
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Department of Biochemistry, Research and Production Enterprise Antigen Co. Ltd., 4 Azerbaev Str., Karasay Area, Abay vill., Almaty, 040509, Kazakhstan
Department of Biology, K. Zhubanov Aktobe regional State University, 34 A. Moldagulova Avenue, Aktobe, 030000, Kazakhstan
Al-Farabi Kazak National University, 71 al-Farabi Avenue, Almaty, 050040, Kazakhstan
Department of Biochemistry
Department of Biology
Al-Farabi Kazak National University
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