Quantitative analysis of hemopoetic chimerism after living-related homogeneous hemopoetic stem cells transplantation
Bexeitova I. Burkitbaev Z. Abdrakhmanova S. Turganbekova A. Turgambayeva A.
October 2021Edizioni Minerva Medica
Gazzetta Medica Italiana Archivio per le Scienze Mediche
2021#180Issue 10539 - 544 pp.
BACKGROUND: Chimerism is a condition where genetically different cells coexist in the same organism. There are several states of chimerism, if after transplantation all blood cells are replaced by donor cells, it is possible to determine the full donor chimerism. It takes time, sometimes quite a long time, to achieve complete donor chimerism. The determination and study of chimerism allowed us to evaluate graft engraftment, as well as the patients condition in the post-transplant period, which was the main goal of our study. METHODS: Modern research methods are the basis for the implementation and optimization of methods for monitoring hematopoietic chimerism in the post-transplantation period of HSC. The definition and study of chimerism allowed us to evaluate the graft engraftment, as well as the patients condition in the post-transplant period, which was the main goal of our study. RESULTS: According to the results of our study on nosological forms, patients with acute myeloid leukemia prevailed in various variants 18 (58.6%). In this group, depending on the degree of HLAcompliance, a large proportion of patients had related ALLO-HSCTfrom donors selected 5/10, which was 8 pairs (44%). According to the results of the study, the information content of the amelogenin locus, which determines the number of pairs that do not coincide by gender, showed 62.5%. CONCLUSIONS: The information content of this locus was observed in 11 of the 14 heterogeneous pairs. In 3 pairs, when the donor-male/recipient-female combination was not observed, and full donor chimerism (99% of the presence of donor DNA) was noted according to the initial data of the definition of hematopoietic chimerism.
Chimerism , Stem cells , Transplantation
Text of the article Перейти на текст статьи
Department of Immunological Tissue Typing (HLA), RSE on PCV Research and Production Center of Transfusiology, Nur-Sultan, Kazakhstan
National Scientific Research Cancer Center, Nur-Sultan, Kazakhstan
RSE on PVC Scientific-Production Center of Transfusiology, Nur-Sultan, Kazakhstan
Department of Immunological Typing, RSE on PVC Scientific-Production Center of Transfusiology, Nur-Sultan, Kazakhstan
Department of Public Health, NJSC Astana Medical University, Nur-Sultan, Kazakhstan
Department of Immunological Tissue Typing (HLA)
National Scientific Research Cancer Center
RSE on PVC Scientific-Production Center of Transfusiology
Department of Immunological Typing
Department of Public Health
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026