Metabolic fluctuations in the human stool obtained from blastocystis carriers and non-carriers
Betts E.L. Newton J.M. Thompson G.S. Sarzhanov F. Jinatham V. Kim M.-J. Popluechai S. Dogruman-Al F. Won E.-J. Gentekaki E. Tsaousis A.D.
December 2021MDPI
Metabolites
2021#11Issue 12
Blastocystis is an obligate anaerobic microbial eukaryote that frequently inhabits the gastrointestinal tract. Despite this prevalence, very little is known about the extent of its genetic diversity, pathogenicity, and interaction with the rest of the microbiome and its host. Although the organism is morphologically static, it has no less than 28 genetically distinct subtypes (STs). Reports on the pathogenicity of Blastocystis are conflicting. The association between Blastocystis and intestinal bacterial communities is being increasingly explored. Nonetheless, similar investigations extending to the metabolome are non-existent.Using established NMR metabolomics protocols in 149 faecal samples from individuals from South Korea (n = 38), Thailand (n = 44) and Turkey (n = 69), we have provided a snapshot of the core metabolic compounds present in human stools with (B+) and without (B−) Blastocystis. Samples included hosts with gastrointestinal symptoms and asymptomatics. A total of nine, 62 and 98 significant metabolites were associated with Blastocystis carriage in the South Korean, Thai and Turkish sample sets respectively, with a number of metabolites increased in colonised groups. The metabolic profiles of B+ and B− samples from all countries were distinct and grouped separately in the partial least squares-discriminant analysis (PLS-DA). Typical inflammation-related metabolites negatively associated with Blastocystis positive samples. This data will assist in directing future studies underlying the involvement of Blastocystis in physiological processes of both the gut microbiome and the host. Future studies using metabolome and microbiome data along with host physiology and immune responses information will contribute significantly towards elucidating the role of Blastocystis in health and disease.
Amino acids , Blastocystis , Inflammation , Metabolite profiles , Metabolomics , NMR , Stool
Text of the article Перейти на текст статьи
Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, United Kingdom
Wellcome Trust Biomolecular NMR Facility, School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, United Kingdom
Faculty of Medicine, Akhmet Yassawi International Kazakh-Turkish University, Turkestan, 161200, Kazakhstan
School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand
Department of Parasitology and Tropical Medicine, Chonnam National University Medical School, Jeollanam-do, Hwasun-gun, Gwangju, 58128, South Korea
Gut Microbiome Research Group, Mae Fah Luang University, Chiang Rai, 57100, Thailand
Division of Medical Parasitology, Department of Medical Microbiology, School of Medicine, Gazi University, Ankara, 06490, Turkey
Department of Laboratory Medicine, Chonnam National University Hwasun Hospital, Jeollanam-do, Hwasun-gun, Gwangju, 58128, South Korea
Laboratory of Molecular and Evolutionary Parasitology
Wellcome Trust Biomolecular NMR Facility
Faculty of Medicine
School of Science
Department of Parasitology and Tropical Medicine
Gut Microbiome Research Group
Division of Medical Parasitology
Department of Laboratory Medicine
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026