A distinct Erwinia amylovora lineage in Kazakhstan associated with a pEA29 transposase mutation
Berdygulova Z.A. Naizabayeva D.A. Dosmagambet Z.M. Rezzonico F. Smits T.H.M. Maltseva E.R.
2025Springer Science and Business Media Deutschland GmbH
Journal of Plant Pathology
2025
Fire blight, caused by Erwinia amylovora, continues to spread across apple-producing regions worldwide. Genomic analyses have revealed that most global isolates belong to the “Widely Prevalent” (WP) lineage, which underwent a bottleneck during its dissemination from North America to Eurasia. Within this lineage, a distinct cluster of strains from Kazakhstan, designated the Za clade, was identified previously. In this study, we report that all Za strains share a single-base deletion in the conserved plasmid pEA29 gene EAMY_3738, encoding a putative ISNCY family transposase. The deletion causes a frameshift and truncation of the protein, predicted to abolish its function. The Za strains also display a distinctive CRISPR genotype, further supporting their peculiar evolutionary status. PCR testing of a set of isolates from 2023 to 2024 showed that the Za lineage is still present as a minor population in the region. This lineage may thus represent a distinct evolutionary variant.
Central Asia , CRISPR genotyping , Fire blight , Frameshift mutation , Genomic adaptation , Microevolution , Molecular epidemiology , Plasmid
Text of the article Перейти на текст статьи
Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan
Almaty Branch of National Center for Biotechnology, Almaty, Kazakhstan
Tethys Scientific Society, Almaty, Kazakhstan
Environmental Genomics and Systems Biology Research Group, Institute for Environment and Natural Resources, Zürich University for Applied Sciences (ZHAW), Wädenswil, Switzerland
Institute of Molecular Biology and Biochemistry
Almaty Branch of National Center for Biotechnology
Tethys Scientific Society
Environmental Genomics and Systems Biology Research Group
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026