Bioinformatics Analysis of the Interaction of miRNAs and piRNAs with Human mRNA Genes Having di-and Trinucleotide Repeats
Belkozhayev A. Niyazova R. Wilson C. Jainakbayev N. Pyrkova A. Ashirbekov Y. Akimniyazova A. Sharipov K. Ivashchenko A.
May 2022MDPI
Genes
2022#13Issue 5
The variability of nucleotide repeats is considered one of the causes of diseases, but their biological function is not understood. In recent years, the interaction of miRNAs and piRNAs with the mRNAs of genes responsible for developing neurodegenerative and oncological diseases and diabetes have been actively studied. We explored candidate genes with nucleotide repeats to predict associations with miRNAs and piRNAs. The parameters of miRNAs and piRNA binding sites with mRNAs of human genes having nucleotide repeats were determined using the MirTarget program. This program defines the start of the initiation of miRNA and piRNA binding to mRNAs, the localization of miRNA and piRNA binding sites in the 5′-untranslated region (5′UTR), coding sequence (CDS) and 3′-untranslated region (3′UTR); the free energy of binding; and the schemes of nucleotide interactions of miRNAs and piRNAs with mRNAs. The characteristics of miRNAs and piRNA binding sites with mRNAs of 73 human genes were determined. The 5′UTR, 3′UTR and CDS of the mRNAs of genes are involved in the development of neurodegenerative, oncological and diabetes diseases with GU, AC dinucleotide and CCG, CAG, GCC, CGG, CGC trinucleotide repeats. The associations of miRNAs, piRNAs and candidate target genes could be recommended for developing methods for diagnosing diseases, including neurodegenerative diseases, oncological diseases and diabetes.
diabetes , gene , miRNA binding , mRNA , neurodegenerative disorders , nucleotide repeats , oncological diseases , piRNA
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Department of Biotechnology, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, 050012, Kazakhstan
School of Human and Life Sciences, Canterbury Christ Church University, Sandwich, CT13 9FF, United Kingdom
Department of Molecular & Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool, Liverpool, L69 3BX, United Kingdom
Department of Public Health, Kazakh-Russian Medical University, Almaty, 050022, Kazakhstan
Center for Bioinformatics and Nanomedicine, Almaty, 050060, Kazakhstan
Higher School of Medicine, Faculty of Medicine and Healthcare, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Department of Biochemistry, Asfendiyarov Kazakh National Medical University, Almaty, 050000, Kazakhstan
Department of Biotechnology
M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry
School of Human and Life Sciences
Department of Molecular & Clinical Cancer Medicine
Department of Public Health
Center for Bioinformatics and Nanomedicine
Higher School of Medicine
Department of Biochemistry
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