The antiproliferative potential and mechanism of action of metformin in MCF-7 cells
Bekezhankyzy Z. Nurzhan S. Berdigaliyev N. Sergazy S. Maulenkul T. Aljofan M.
2023Newlands Press Ltd
Future Science OA
2023#9Issue 5
Aim: The current study aimed to investigate the potential antiproliferative activity of metformin, the effective concentration range, and the mechanism of action. Materials and methods: Human breast cancer cells, MCF-7 were treated with a serial dilution of metformin (10-150 ?M) for 24 and 48 h. Potential antiproliferative activity of metformin and its ability in inducing cellular apoptosis and autophagy were also investigated. Results: Metformin inhibited MCF-7 proliferation in a concentration and time dependent manner, with 80 ?M as the most effective concentration. Compared with nontreated cells, metformin induced significant levels of autophagy and apoptosis, which were confirmed by the reduction of mTOR and BCL-2 protein expression. Conclusion: The study confirms the antiproliferative activity of metformin, which may likely occur through AMPK signaling pathway.
antiproliferative , apoptosis , autophagy , breast cancer , metformin
Text of the article Перейти на текст статьи
Department of Biomedical Sciences, School of Medicine, Nazarbayaev University, Astana, 010000, Kazakhstan
National Laboratory Astana, Nazarbayev University, Astana, 010000, Kazakhstan
Khoja Akhmet Yassawi International Kazakh-Turkish University Turkistan, Turkistan, 161211, Kazakhstan
Department of Biomedical Sciences
National Laboratory Astana
Khoja Akhmet Yassawi International Kazakh-Turkish University Turkistan
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026