Growth and hydrogen production by Escherichia coli during utilization of sole and mixture of sugar beet, alcohol, and beer production waste
Bekbayev K. Mirzoyan S. Toleugazykyzy A. Tlevlessova D. Vassilian A. Poladyan A. Trchounian K.
January 2024Springer Science and Business Media Deutschland GmbH
Biomass Conversion and Biorefinery
2024#14Issue 1909 - 919 pp.
Escherichia coli anaerobically utilize various carbon sources and produce hydrogen (H2) as fermentation end product. Twofold diluted mixture of 10% distiller’s grains (DG), 4% brewer’s spent grains (BSG), and 10% sugar beet molasses (SB) are favorable for enhanced H2 production compared to the single wastes. In wild type cells, H2 production was prolonged till ~ 120 h compared to single waste while in multiple mutant H2 production was determined till ~ 192 h. Specific growth rate was highest in wild type cells in single but not mixed carbon sources reaching to 0.723 h−1 with 20-fold dilution of SB utilization. Cumulative H2 production was ~ 2.7-fold higher in multiple mutant when twofold diluted mixed waste of SB, BSG, and DG was applied compared to wild type reaching to 215 mL. The obtained data can be applied in utilization of agro-industrial waste for production of biomass and bio-H2.
Agro-industrial waste , Biomass and bioenergy , Dark fermentation
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Department of Food Technology and Processing Products, Technical Faculty, S. Seifullin Kazakh Agro-Technical University, 62 Zhenis avenue, Nur-Sultan, Z11F9K5, Kazakhstan
Scientific-Research Institute of Biology, Faculty of Biology, Yerevan State University, Yerevan, 0025, Armenia
Department of Biochemistry, Microbiology and Biotechnology, Faculty of Biology, Yerevan State University, 1 A. Manoogian Str, Yerevan, 0025, Armenia
Microbial Biotechnologies and Biofuel Innovation Center, Yerevan State University, Yerevan, 0025, Armenia
Department of Technology of Food Products, Almaty Technological University, Almaty, 050012, Kazakhstan
Department of Ecology and Nature Protection, Faculty of Biology, Yerevan State University, Yerevan, 0025, Armenia
Department of Food Technology and Processing Products
Scientific-Research Institute of Biology
Department of Biochemistry
Microbial Biotechnologies and Biofuel Innovation Center
Department of Technology of Food Products
Department of Ecology and Nature Protection
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