Phenotypic and genotypic resistance to antibiotics in Staphylococcus aureus strains isolated from cattle milk in Northern Kazakhstan
Baymenov B.M. Bulashev A.K. Chuzhebayeva G.D. Aliyeva G.K. Beishova I.S. Kokanov S.K. Raketsky V.A.
September 2023Veterinary World
Veterinary World
2023#16Issue 91815 - 1820 pp.
Background and Aim: Staphylococcus aureus is the most frequent and ubiquitous cause of mastitis in cows. In recent decades, antibiotic resistance has rapidly spread among infectious disease pathogens in Kazakhstan and globally. This study examined the phenotypic and genotypic resistance of S. aureus strains obtained from cattle milk to antibiotics. Materials and Methods: In 2021 and 2022, 675 cow milk samples were collected from 16 dairy farms in Northern Kazakhstan. Staphylococcus aureus was identified using culture and biochemical methods. The nature of antibiotic resistance was determined by the disk diffusion (DD) method. The distribution of antibiotic resistance genes was determined by polymerase chain reaction. Results: Among the obtained S. aureus isolates, high levels of resistance to β-lactam antibiotics (100%), tetracyclines (95.4%), fluoroquinolones (95.4%), and macrolides (60.92%) were observed. Meanwhile, the lowest levels of resistance were identified for sulfonamides (21.84%) and aminoglycosides (27.59%). All the obtained isolates were positive for the nuc gene encoding thermonuclease. The blaZ, ermC, and tetK genes were detected in 45.9%, 77%, and 83.9% of the studied S. aureus isolates, respectively. Conclusion: The results indicate a high prevalence of antibiotic resistance in S. aureus isolated from cows with clinical and subclinical forms of mastitis in Northern Kazakhstan. In addition, the prevalence of resistance was higher when evaluated by the DD method than when detecting the specific antibiotic resistance genes blaZ, tetK, and ermC, indicating the need for deeper analysis of the phenotypic and genetic determinants of antibiotic resistance.
antibiotic resistance genes , cow milk , polymerase chain reaction , Staphylococcus aureus strains
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Research Institute of Applied Biotechnology, A. Baitursynov Kostanay Regional University, Kostanai, Kazakhstan
Department of Microbiology and Biotechnology, S. Seifullin Kazakh Agro Technical Research University, Astana, Kazakhstan
Testing Center, West Kazakhstan Agrarian and Technical University Named after Zhangir Khan, Uralsk, Kazakhstan
Department of Veterinary Medicine, A. Baitursynov Kostanay Regional University, Kostanai, Kazakhstan
Research Institute of Applied Biotechnology
Department of Microbiology and Biotechnology
Testing Center
Department of Veterinary Medicine
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