Isolation and Screening of the Novel Multi-Trait Strains for Future Implications in Phytotechnology
Batykova Z. Pidlisnyuk V. Kistaubayeva A. Ust’ak S. Savitskaya I. Saidullayeva L. Mamirova A.
August 2025Multidisciplinary Digital Publishing Institute (MDPI)
Microorganisms
2025#13Issue 8
Plant growth-promoting rhizobacteria (PGPRs) colonise the rhizosphere and root surfaces, enhancing crop development through a variety of mechanisms. This study evaluated microbial strains isolated from Triticum aestivum L. for key plant growth-promoting traits, including indole-3-acetic acid (IAA) production, phosphate and zinc (Zn) solubilisation, nitrogen (N2) fixation, and antifungal activity. Among 36 isolates, 3 (AS8, AS23, AS31) exhibited strong growth-promoting potential. IAA production, citrate assimilation, carbohydrate fermentation, and catalase activity were observed to a comparable extent among the selected strains. AS8 showed the highest protease, lipase, and amylolytic activity, while AS23 demonstrated superior phosphate and Zn solubilisation. Notably, AS31 emerged as the most promising multi-trait isolate, exhibiting the highest levels of IAA production, N2 fixation, antifungal activity against five phytopathogens (Fusarium graminearum, F. solani, F. oxysporum, Pythium aphanidermatum, and Alternaria alternata), potentially linked to its hydrogen sulphide (H2S) production, and cellulolytic activity. Molecular identification based on 16S rRNA gene sequencing revealed the isolates as Stenotrophomonas indicatrix AS8, Pantoea agglomerans AS23, and Bacillus thuringiensis AS31. Seed germination assays confirmed the plant growth-promoting efficacy of these PGPR strains, with vigour index increases of up to 43.4-fold. Given their positive impact on seed germination and significant Zn-solubilising abilities, the selected strains represent promising candidates for use as bio-inoculants, offering a sustainable and eco-friendly strategy to enhance agricultural productivity in nutrient-deficient soils. Future research should validate the efficacy of these PGPR strains under pot conditions to confirm their potential for practical agricultural applications.
antifungal activity , indole-3-acetic acid production , microbial isolates , P and Zn solubilisation , PGPR inoculants , seed germination
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Department of Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty, 050040, Kazakhstan
Department of Environmental Chemistry & Technology, Faculty of Environment, Jan Evangelista Purkyně University, Pasteurova 1, Ústí nad Labem, 400 96, Czech Republic
Czech Agrifood Research Centre, Chomutov, 430 01, Czech Republic
Department of Biotechnology, Auezov South Kazakhstan University, Tauke Khan 5, Shymkent, 160012, Kazakhstan
Department of Biotechnology
Department of Environmental Chemistry & Technology
Czech Agrifood Research Centre
Department of Biotechnology
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