High-risk human papillomaviruses L1 gene isolates identified in Western Kazakhstan
Balmagambetova S.K. Zholdybayeva E.V. Zavalennaya O.V. Amanzholkyzy A. Kononets V.I. Zharmakhanova G.M. Mussin N.M. Zhamaliyeva L.M. Kereyeva N.M.
2024National Scientific Medical Center
Journal of Clinical Medicine of Kazakhstan
2024#21Issue 126 - 34 pp.
Kazakhstani researchers reported a significant prevalence of highly carcinogenic human papillomavirus types in the country. The article aimed to present HPV L1 gene sequencing developments in women affected with cervical cancer throughout the western part of Kazakhstan with provided findings on the geographic pathways of obtained isolates. Methods. The HPV L1 gene was amplified using the consensus primers MY09HPV 5’-CGTCCMARRGGAWACTGATC-3’ and MY11HPV 5’ – GCMCAGGGWCATAAYAATGG-3’. The purified DNA was used as the target for direct nucleotide sequencing. Phylogenetic analyses were conducted using the MegAlign program from the LASERGENE software package (version 6.0; DNA star, Madison, WI) and MEGA version 5.0 software. A multiple alignment was created through Clustal W software, and the neighbor-joining method was used to construct the phylogenetic tree. Results. Of 70 HPV samples transported to the Astana shared laboratory for gene L1 sequencing, only ten appeared fit to obtain isolates (14.3%). The viral load of the samples ranged from 3.3 to 8.2, and the range of DNA concentration was from 8.16 to 69.6 ng/uL. Not yet registered in the world genebank, a unique HPV16 Kazakhstani isolate with its own branch was revealed in Aktobe. An isolate of potentially carcinogenic HPV53 forming a remote cluster with KF436822/1, KU951264.1 – Southwest China, and 97% identity with EU056643.1 – Ireland, and acted as a single agent for invasive cervical cancer was identified. In general, the sequencing findings indicate the variety of ways for HPV pervasion into the western region of Kazakhstan: North and South America, Europe, and Asia. The study was recorded in the ISRCTN registry, No. 7154910, 02/01/2018.
cervical cancer , gene L1 , gene sequencing , Human papillomavirus , isolates , western Kazakhstan
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Department of Oncology, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Shared laboratory, National Center for Biotechnology, Astana, Kazakhstan
PCR laboratory, Scientific-Production Center, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Department of Physiology, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Department of Natural Science Disciplines, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Department of Transplantology, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Department of Family Medicine, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Department of Oncology
Shared laboratory
PCR laboratory
Department of Physiology
Department of Natural Science Disciplines
Department of Transplantology
Department of Family Medicine
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