Radiofrequency Electromagnetic and Pulsed Magnetic Fields Protected the Kidney Against Lipopolysaccharide-Induced Acute Systemic Inflammation, Oxidative Stress, and Apoptosis by Regulating the IL-6/HIF1α/eNOS and Bcl2/Bax/Cas-9 Pathways
Balci Ç. Özcan M.S. Aşci H. Karabacak P. Kuruşçu O. Taner R. Özmen Ö. Tepebaşi M.Y. İlhan İ. Çömlekçi S.
February 2025Multidisciplinary Digital Publishing Institute (MDPI)
Medicina (Lithuania)
2025#61Issue 2
Background/Objectives: Sepsis-associated acute kidney injury caused by lipopolysaccharide (LPS) is related to hypoxia, amplification of the inflammatory response, oxidative stress, mitochondrial dysfunction, and apoptosis. This study aims to explore the protective effects of a radiofrequency electromagnetic field (RF-EMF) and a pulsed magnetic field (PMF) on acute kidney injury in rats. Materials and methods: Forty female Wistar albino rats were randomly divided into five groups (each containing eight rats): control, LPS, RF-EMF, PMF, and RF-EMF + PMF groups. Six hours after LPS application, blood and tissues were removed for histopathological, immunohistochemical, biochemical, and genetic analysis. Results: Histopathological findings, caspase-3, inducible nitric oxide synthase and tumor necrosis factor-alpha immunoexpressions, total oxidant status and oxidative stress index levels, and interleukin-6, hypoxia-inducible factor alpha, Bcl-2-associated X protein, and caspase 9 gene expression in kidney tissue and blood urine nitrogen and creatinine levels in blood were increased, whereas endothelial nitric oxide synthase and B-cell lymphoma 2 gene expression were decreased in the LPS groups. Both RF-EMF and PMF reversed all these findings and recovered renal tissues. Conclusions: Noninvasive, nontoxic, low-cost PMF and RF-EMF, both single and combined, have been demonstrated to have renoprotective anti-inflammatory, antioxidant, and antiapoptotic effects.
acute kidney injury , inflammation , pulsed magnetic field (PMF) , radiofrequency electromagnetic field (RF-EMF) , sepsis
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Department of Anesthesiology and Reanimation, Faculty of Medicine, Suleyman Demirel University, Isparta, 32260, Kazakhstan
Department of Pharmacology, Faculty of Medicine, Suleyman Demirel University, Isparta, 32260, Kazakhstan
Department of Bioengineering, Institute of Science, Suleyman Demirel University, Isparta, 32260, Kazakhstan
Department of Pathology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, 15200, Turkey
Department of Medical Genetics, Faculty of Medicine, Suleyman Demirel University, Isparta, 32260, Kazakhstan
Department of Biochemistry, Faculty of Medicine, Suleyman Demirel University, Isparta, 32260, Kazakhstan
Department of Electronics and Communication Engineering, Faculty of Engineering, Suleyman Demirel University, Isparta, 32260, Kazakhstan
Department of Anesthesiology and Reanimation
Department of Pharmacology
Department of Bioengineering
Department of Pathology
Department of Medical Genetics
Department of Biochemistry
Department of Electronics and Communication Engineering
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