Characterization of atypical pathogenic Aeromonas salmonicida isolated from a diseased Siberian sturgeon (Acipenser baerii)
Bakiyev S. Smekenov I. Zharkova I. Kobegenova S. Sergaliyev N. Absatirov G. Bissenbaev A.
July 2023Elsevier Ltd
Heliyon
2023#9Issue 7
Acipenser baerii (Siberian sturgeon) is native to Kazakhstan and is currently endangered and is listed within the first class of protected animals. Sturgeon aquaculture is becoming an important tool for the recovery of this endangered species. Nonetheless, diseases involving typical symptoms of skin ulceration and systemic bacterial hemorrhagic septicemia have occurred in cultured A. baerii on a fish farm located in Western Kazakhstan. In this study, an infectious strain of bacteria isolated from an ulcer of diseased A. baerii was identified as Aeromonas salmonicida (strain AB001). This identification involved analyses of 16S rRNA, gyrB, rpoD, and flaA genes’ sequences. Even though strain AB001 belongs to A. salmonicida, it exhibited noticeable mobility and growth at temperatures of ≥37 °C. Profiling of virulence genes uncovered the presence of seven such genes related to pathogenicity. Antibiotic sensitivity testing showed that the strain is sensitive to aminoglycosides, amphenicols, nitrofurans, quinolones, and tetracyclines. Half-lethal doses (LD50) of strain AB001 for Oreochromis mossambicus and A. baerii were determined: respectively 1.7 × 108 and 7.2 × 107 colony-forming units per mL. The experimentally induced infection revealed that strain AB001 causes considerable histological lesions in O. mossambicus, including tissue degeneration, necrosis, and hemorrhages of varied severity.
Acipenser baerii , Aeromonas salmonicida , Antibiotic resistance , Oreochromis mossambicus , Pathogenicity , Virulence gene
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Department of Molecular Biology and Genetics, Faculty of Biology and Biotechnology, al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Scientific Research Institute of Biology and Biotechnology Problems, al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Department of Biodiversity and Bioresources, Faculty of Biology and Biotechnology, al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Makhambet Utemisov West Kazakhstan University, Uralsk, 090000, Kazakhstan
West Kazakhstan Innovation and Technological University, Uralsk, 090000, Kazakhstan
Department of Molecular Biology and Genetics
Scientific Research Institute of Biology and Biotechnology Problems
Department of Biodiversity and Bioresources
Makhambet Utemisov West Kazakhstan University
West Kazakhstan Innovation and Technological University
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