MRI Tracking of Marine Proliferating Cells In Vivo Using Anti-Oct4 Antibody-Conjugated Iron Nanoparticles for Precision in Regenerative Medicine
Baghban N. Khoradmehr A. Afshar A. Jafari N. Zendehboudi T. Rasekh P. Abolfathi L.G. Barmak A. Mohebbi G. Akmaral B. Askerovich K.A. Maratovich M.N. Azari H. Assadi M. Nabipour I. Tamadon A.
February 2023MDPI
Biosensors
2023#13Issue 2
Marine invertebrates are multicellular organisms consisting of a wide range of marine environmental species. Unlike vertebrates, including humans, one of the challenges in identifying and tracking invertebrate stem cells is the lack of a specific marker. Labeling stem cells with magnetic particles provides a non-invasive, in vivo tracking method using MRI. This study suggests antibody-conjugated iron nanoparticles (NPs), which are detectable with MRI for in vivo tracking, to detect stem cell proliferation using the Oct4 receptor as a marker of stem cells. In the first phase, iron NPs were fabricated, and their successful synthesis was confirmed using FTIR spectroscopy. Next, the Alexa Fluor anti-Oct4 antibody was conjugated with as-synthesized NPs. Their affinity to the cell surface marker in fresh and saltwater conditions was confirmed using two types of cells, murine mesenchymal stromal/stem cell culture and sea anemone stem cells. For this purpose, 106 cells of each type were exposed to NP-conjugated antibodies and their affinity to antibodies was confirmed by an epi-fluorescent microscope. The presence of iron-NPs imaged with the light microscope was confirmed by iron staining using Prussian blue stain. Next, anti-Oct4 antibodies conjugated with iron NPs were injected into a brittle star, and proliferating cells were tracked by MRI. To sum up, anti-Oct4 antibodies conjugated with iron NPs not only have the potential for identifying proliferating stem cells in different cell culture conditions of sea anemone and mouse cell cultures but also has the potential to be used for in vivo MRI tracking of marine proliferating cells.
cell proliferation , magnetic nanoparticle , marine , MRI , tracking
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The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, 7514633196, Iran
PerciaVista R&D Co, Shiraz, 7167683745, Iran
MRI Department, Heart Hospital of Bushehr, Bushehr University of Medical Sciences, Bushehr, 7514633196, Iran
Food Laboratory, Bushehr University of Medical Sciences, Bushehr, 7518759577, Iran
Department for Scientific Work, West Kazakhstan Marat Ospanov Medical Unversity, Aktobe, 030012, Kazakhstan
General Surgery, West-Kazakhstan Medical University Named after Marat Ospanov, Aktobe, 030012, Kazakhstan
Nuclear Medicine and Molecular Imaging Research Center, School of Medicine, Bushehr University of Medical Sciences, Bushehr, 7514633196, Iran
The Persian Gulf Marine Biotechnology Research Center
PerciaVista R&D Co
MRI Department
Food Laboratory
Department for Scientific Work
General Surgery
Nuclear Medicine and Molecular Imaging Research Center
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