Detection of Molecular Markers of Potato Virus X Resistance Genes in the Potato Gene Pool of Kazakhstan
Azhimakhan M. Khassanov V. Vologin S. Hu B. Tokbergenova Z. Amirgazin A. Shevtsov A. Beisembina B.
March-April 2025Unique Scientific Publishers
International Journal of Agriculture and Biosciences
2025#14Issue 2201 - 208 pp.
The study aimed to identify and characterize the presence of molecular markers associated with potato virus X resistance genes (Rx1, Rx2, and Nb) in the gene pool of Kazakhstan. During the study, 71 potato varieties and breeding lines were analyzed using the SCAR molecular markers and markers GM 637 and GM 339 for the Nb gene. DNA was extracted from leaf tissues and analyzed using a polymerase chain reaction to identify the presence of these markers. Artificial potato virus X inoculation was performed under controlled conditions in a phytotron followed by ELISA tests to confirm the infection status. The study identified SCAR markers for the Rx1 gene in 25% of the samples and for the Rx2 gene, in 77%. Markers of the Nb gene were found in 68% of the samples. 15 varieties showed extreme resistance to potato virus X, as evidenced by the absence of symptoms and negative ELISA results, while three varieties showed resistance similar to tolerance, where potato virus X was detected in leaf tissue without symptoms. The characterized varieties and breeding lines of the potato gene pool of Kazakhstan, which have an extreme type of resistance to potato virus X, can be included in breeding programs to create new virus-resistant potato varieties in the future.
Breeding line , Infection , Molecular marker , Potato virus X , Resistance
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Saken Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan
Tatar Research Institute of Agriculture, Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russian Federation
Leling Xisen Potato Industry Group Co. Ltd., Laoling, China
Kazakh Scientific Research Institute of Fruit Growing and Viticulture, Almaty, Kazakhstan
National Center for Biotechnology, Astana, Kazakhstan
Saken Seifullin Kazakh Agrotechnical Research University
Tatar Research Institute of Agriculture
Leling Xisen Potato Industry Group Co. Ltd.
Kazakh Scientific Research Institute of Fruit Growing and Viticulture
National Center for Biotechnology
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