Phylogenetic analysis of a 2024 Sheeppox virus isolate from the Almaty region of Kazakhstan and investigation of its pathogenicity in merino sheep
Azanbekova M. Mambetaliyev M. Valiyeva A. Kozhabergenov N. Aldayarov N. Kilibayev S. Usserbayev B. Tuyskanova M. Kadyrova B. Myrzakhmetova B. Kutumbetov L. Chervyakova O. Nurabayev S. Berdikulov M. Kerimbayev A. Rsaliyev A. Abduraimov Y. Zhugunissov K.
2025Frontiers Media SA
Frontiers in Veterinary Science
2025#12
Introduction: Sheeppox virus (SPPV) is a significant pathogen that affects small ruminants and causes substantial economic losses. It is essential to perform detailed molecular and pathogenic characterization of field isolates to control the disease and develop prevention strategies. Methods: The SPPV isolate “Sheeppox/KZ/Targap/2024” was obtained in 2024 from a diseased lamb during an outbreak in Targap village in the Almaty region of Kazakhstan. The isolate was passaged three times in lamb kidney-cell culture, and the 50% tissue culture infectious dose (TCID50) assay indicated a titer of 5.33 ± 0.08 log10 TCID50/mL. Identification was performed using polymerase chain reaction (PCR) and whole-genome sequencing, and the complete genome was submitted to GenBank (accession number PV434148). Experimental infection studies were conducted with Merino sheep. Results: The virus activity was 4.75 ± 0.02 log10 ID50/mL. The virus neutralization test showed 100% seroconversion by day 9 post-infection and maximum antibody titers (1:32) by day 21. ELISA confirmed that there was a strong immune response (>90% seropositivity). PCR detected viral DNA by day 5 post-infection in most tissues. Necropsy revealed typical pathological sheeppox lesions in the lungs, spleen, lymph nodes, and skin. Histopathological analysis demonstrated acute-stage features including massive cellular infiltration, vasculitis, edema, and pox lesions. Conclusion: The findings confirm previously known characteristics of SPPV and provide new insights into the molecular and pathogenic properties of the “Sheeppox/KZ/Targap/2024” isolate. Copyright
histopathological changes , pathogenicity , pathological examination , phylogenetic analysis , sequencing , Sheeppox , virus
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Research Institute for Biological Safety Problems, National Holding “QazBioPharm”, Guardeyskiy, Kazakhstan
Kyrgyz-Turkish Manas University, Bishkek, Kyrgyzstan
National Reference Center for Veterinary, Astana, Kazakhstan
National Holding “QazBioPharm”, Astana, Kazakhstan
Research Institute for Biological Safety Problems
Kyrgyz-Turkish Manas University
National Reference Center for Veterinary
National Holding “QazBioPharm”
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