Optimization of the process of cryopreservation of reproductive cells in male pikeperch, Sander lucioperca
Assylbekova A.S. Barinova G.K. Syzdykov K.N. Mussina A.D. Aubakirova G.A. Makhanbetova A.B. Kuanchaleyev Z.B.
January 2025University of Guilan
Caspian Journal of Environmental Sciences
2025#23Issue 1225 - 232 pp.
The process for cryopreservation of pikeperch sperm was studied as part of the research. The purpose of these studies is to assess the effect of different concentrations of DMSO cryoprotectant during cryopreservation of pikeperch sperm on the sperm quality indicators. The study of sperm quality was carried out according to generally accepted methods, in which the following indicators were determined: the concentration of fresh sperm, the motility of spermatozoa of fresh and defrosted sperm, and the sperm activity time. In the course of the research, we studied the effect of cryoprotectant concentration on the motility of spermatozoa, in which good results were obtained when using DMSO with a concentration of 10%, which was 68.2 ± 2.24% in the first group and 58.8 ± 2.16% in the second group comparing to DMSO at 20% (p < 0.01). The highest motility rate of spermatozoa was observed by sperm freezing with equilibration of 68.2 ± 2.24% in the first group and 58.8 ± 2.16% in the second group compared to equilibration (p < 0.01). When studying the sperm activity time in two groups, high results were observed when frozen with DMSO 10% cryoprotectant at 883.4 ± 15.12 s and 799.4 ± 10.32 s compared to DMSO 20% cryoprotectant, respectively (p<0.05). Of the two groups studied, the group with hormonal stimulation showed the best results in terms of sperm quality. Thus, when studying the protocol for cryopreservation of pikeperch sperm, we found that DMSO cryoprotectant at a concentration of 10% has a positive effect on the quality of deforested sperm.
Cryopreservation , Cryoprotector , Pikeperch , Reproductive cells , Sperm
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Department of Hunting and Fisheries, S.Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan
Department of Biodiversity and Bioresources, al-Farabi Kazakh National university, Almaty, Kazakhstan
Joint-Stock Company Republican Center for Breeding in Animal Husbandry Asyl Tulik, Kosshy, Kazakhstan
Department of Hunting and Fisheries
Department of Biodiversity and Bioresources
Joint-Stock Company Republican Center for Breeding in Animal Husbandry Asyl Tulik
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