Development of a Bacterial Lysate from Antibiotic-Resistant Pathogens Causing Hospital Infections
Anuarbekova S. Sadykov A. Amangeldinova D. Kanafina M. Sharova D. Alzhanova G. Nurgaliyeva R. Jumagaziyeva A. Tynybayeva I. Zhumakaeva A. Rsaliyev A. Abduraimov Y. Kanafin Y.N.
August 2025Multidisciplinary Digital Publishing Institute (MDPI)
Microorganisms
2025#13Issue 8
Biotechnological research increasingly focuses on developing new drugs to counter the rise of antibiotic-resistant strains in hospitals. This study aimed to create bacterial lysates from antibiotic-resistant pathogens isolated from patients and medical instruments across hospital departments. Identification was performed based on morphological, cultural, and biochemical characteristics, as well as 16S rRNA gene sequencing using the BLAST algorithm. Strain viability was assessed using the Miles and Misra method, while sensitivity to eight antibacterial drug groups and biosafety between cultures were evaluated using agar diffusion. From 15 clinical sources, 25 pure isolates were obtained, and their phenotypic and genotypic properties were studied. Carbohydrate fermentation testing confirmed that the isolates belonged to the genera Escherichia, Citrobacter, Klebsiella, Acinetobacter, Pseudomonas, Staphylococcus, Haemophilus, and Streptococcus. The cultures exhibited good viability (109–1010 CFU/mL) and compatibility with each other. Based on prevalence and clinical significance, three predominant hospital pathogens (Klebsiella pneumoniae 12 BL, Pseudomonas aeruginosa 3 BL, and Acinetobacter baumannii 24 BL) were selected to develop a bacterial lysate consortium. Lysates were prepared with physical disruption using a French press homogenizer. The resulting product holds industrial value and may stimulate the immune system to combat respiratory pathogens prevalent in Kazakhstan’s healthcare settings.
antibiotic resistance , bacterial lysate , clinical isolates , French press homogenization , respiratory pathogens
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Scientific and Analytical Center “Biomedpreparat” LLP, Stepnogorsk, 021500, Kazakhstan
Scientific Center for Anti-Infectious Drugs, Almaty, 050000, Kazakhstan
National Holding “QazBioPharm”, Astana, 010000, Kazakhstan
Faculty of Natural Sciences, L.N. Gumilyov Eurasian National University, Astana, 010000, Kazakhstan
Scientific and Analytical Center “Biomedpreparat” LLP
Scientific Center for Anti-Infectious Drugs
National Holding “QazBioPharm”
Faculty of Natural Sciences
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