Selection of rice breeding lines for resistance to biotic and abiotic stresses
Seleção de linhas de melhoramento de arroz para resistência a estresses bióticos e abióticos
Amirova A. Usenbekov B. Berkimbay K. Mynbayeva D. Atabayeva S. Baiseitova G. Meldebekova A. Zhunusbayeva Z. Kenzhebayeva S. Mukhambetzhanov S.
2024Instituto Internacional de Ecologia
Brazilian Journal of Biology
2024#84
Rice (Oryza sativa L.) grown in many countries around the world with different climatic conditions and a huge number of environmental stresses, both biotic (fungi, bacteria, viruses, insects) and abiotic (cold, drought, salinity) limit rice productivity. In this regard, breeders and scientists are trying to create rice lines that are resistant to multiple stresses. The aim of this work was to screen and select cold and blast resistant rice breeding lines (RBLs) using molecular markers. Molecular screening of RBLs and parental varieties to cold tolerance was carried out using markers RM24545, RM1377, RM231 and RM569 associated with QTLs (qPSST-3, qPSST-7, qPSST-9). It was discovered that the presence of three QTLs characterizes the cold resistance of studied genotypes, and the absence of one of them leads to cold sensitivity. As a result, 21 cold-resistant out of the 28 studied RBLs were identified. These cold resistant 21 RBLs were further tested to blast resistance using markers Pi-ta, Pita3, Z56592, 195R-1, NMSMPi9-1, TRS26, Pikh MAS, MSM6, 9871.T7E2b, RM224 and RM1233. It was revealed that 16 RBLs from 21 studied lines contain 5-6 blast resistance genes. In accordance with the blast resistance strategy, the presence of 5 or more genes ensures the formation of stable resistance to Magnaporthe oryzae. Thus, 16 lines resistant to multiple stresses, such as cold and blast disease were developed. It should be noted that 6 of these selected lines are high-yielding, which is very important in rice breeding program. These RBLs can be used in breeding process as starting lines, germplasm exchange as a source of resistant genes for the development of new rice varieties resistant to multiple stress factors.
blast resistance , cold resistance , Oryza sativa L , rice breeding lines
Text of the article Перейти на текст статьи
Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan
Al-Farabi Kazakh National University, Faculty of Biology and Biotechnology, Department of Molecular Biology and Genetics, Almaty, Kazakhstan
Al-Farabi Kazakh National University, Faculty of Biology and Biotechnology, Department of Biotechnology, Almaty, Kazakhstan
Kazakh National Agrarian University, Almaty, Kazakhstan
Institute of Plant Biology and Biotechnology
Al-Farabi Kazakh National University
Al-Farabi Kazakh National University
Kazakh National Agrarian University
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026