Distribution of Runs of Homozygosity and Their Relationship with Candidate Genes for Productivity in Kazakh Meat–Wool Sheep Breed
Amandykova M. Akhatayeva Z. Kozhakhmet A. Kapassuly T. Orazymbetova Z. Yergali K. Khamzin K. Iskakov K. Dossybayev K.
November 2023Multidisciplinary Digital Publishing Institute (MDPI)
Genes
2023#14Issue 11
Increasing the fertility of sheep remains one of the crucial issues of modern sheep breeding. The Kazakh meat–wool sheep is an excellent breed with high meat and wool productivity and well adapted to harsh conditions. Nowadays, runs of homozygosity (ROHs) are considered a suitable approach for studying the genetic characteristics of farm animals. The aims of the study were to analyze the distribution of ROHs, describe autozygosity, and detect genomic regions with high ROH islands. In this study, we genotyped a total of 281 Kazakh meat–wool sheep using the Illumina iScan® system (EquipNet, Canton, MA, USA) via Ovine SNP50 BeadChip array. As a results, a total of 15,069 ROHs were found in the three Kazakh meat–wool sheep populations. The mean number of ROH per animal across populations varied from 40.3 (POP1) to 42.2 (POP2) in the category 1+ Mb. Furthermore, the number of ROH per animal in ROH1–2 Mb were much higher than ROH2–4 Mb and ROH8–16 Mb in the three sheep populations. Most of individuals had small number of ROH>16 Mb. The highest and lowest genomic inbreeding coefficient values were observed in POP2 and POP3, respectively. The estimated FROH presented the impact that recent inbreeding has had in all sheep populations. Furthermore, a set of interesting candidate genes (BMP2, BMPR2, BMPRIB, CLOCK, KDM2B, TIAM1, TASP1, MYBPC1, MYOM1, and CACNA2D1), which are related to the productive traits, were found. Collectively, these findings will contribute to the breeding and conservation strategies of the Kazakh meat–wool sheep breed.
fertility , genes , inbreeding , Kazakh meat–wool sheep , ROH
Text of the article Перейти на текст статьи
Laboratory of Animal Genetics and Cytogenetics, Institute of Genetics and Physiology SC MSHE RK, Al-Farabi Ave. 93, Almaty, 050060, Kazakhstan
Department of Molecular Biology and Genetics, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi Ave. 71, Almaty, 050042, Kazakhstan
Laboratory of Molecular Genetics, Kazakh Research Institute of Livestock and Fodder Production, Zhandosov Str. 51, Almaty, 050035, Kazakhstan
Laboratory of Animal Genetics and Cytogenetics
Department of Molecular Biology and Genetics
Laboratory of Molecular Genetics
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026