Modulation of insulin gene expression with crispr/cas9-based transcription factors
Alzhanuly B. Mukhatayev Z.Y. Botbayev D.M. Ashirbekov Y. Katkenov N.D. Dzhaynakbaev N.T. Sharipov K.O.
2021Scientific Foundation SPIROSKI
Open Access Macedonian Journal of Medical Sciences
2021#9Issue A876 - 881 pp.
BACKGROUND: The discovery and use of CRISPR/Cas9 technology have enabled researchers throughout the globe to continuously edit genomes for the benefit of science and medicine. Diabetes type I is one field of medicine where CRISPR/Cas9 has a strong potential for cell therapy development. The long-lasting paucity of healthy cells for clinical transplantation into diabetic patients has led to the search of new methods for producing β-cells from other human cell types. Embryonic stem cells are being studied worldwide as one most promising solution of this need. AIM: The aim of the study is to check the feasibility of modulating human insulin transcription using CRISPR/Cas9-based synthetic transcription regulation factors. METHODS: A new approach for creating potential therapeutic donor cells with enhanced and suppressed insulin production based on one of the latest achievements of human genome editing was developed. RESULTS: Both synthetic transcription activator (VP64) and transcription repressor (KRAB) proteins were shown to function adequately well as a part of the whole CRISPR/Cas9-based system. CONCLUSION: We claim that our results have a lot to offer and can bring light to many studies where numerous laboratories are struggling on to treat this disease.
CRISPR/dCas9 system , Diabetes type I , Embryonic stem cells , HEK293 cells
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Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan
Department of Molecular Biology and Genetics, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan
Department of Research and Development, Almaty Management University, Almaty, Kazakhstan
Zhangir Khan University, Uralsk, Kazakhstan
Kazakh-Russian Medical University, Almaty, Kazakhstan
Aitkhozhin Institute of Molecular Biology and Biochemistry
Department of Molecular Biology and Genetics
Department of Research and Development
Zhangir Khan University
Kazakh-Russian Medical University
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