Retrospective evaluation of viral respiratory tract infections in a university hospital in Ankara, Turkey (2016-2019)
Altay-Kocak A. Sarzhanova S. Tapisiz A. Dizbay M. Basustaoglu A. Bozdayi G.
May 2022Journal of Infection in Developing Countries
Journal of Infection in Developing Countries
2022#16Issue 5857 - 863 pp.
Introduction: Viruses are responsible for two-thirds of all acute respiratory tract infections. This study aims to retrospectively detect respiratory tract viruses in patients from all age groups who visited the hospital. Methodology: A total of 1592 samples from 1416 patients with respiratory tract symptoms were sent from several clinics to the Molecular Microbiology Laboratory at Gazi University Hospital from February 2016 to January 2019. Nucleic acid extraction from nasopharyngeal swabs, throat swabs or bronchoalveolar lavage (BAL) samples sent to our laboratory was done using a commercial automated system. Extracted nucleic acids were amplified by a commercial multiplex-real time Polymerase Chain Reaction (PCR) method, which can detect 18 viral respiratory pathogens. Results: Among 1592 samples, 914 (57.4%) were positive for respiratory viruses. The most prevalent were rhinovirus (25.2%) and influenza A virus (12.1%), the least prevalent was the bocavirus (2.6%). Rhinovirus was the most detected as a single agent (21.2%, 194/914) among all positive cases, followed by coronavirus (9.3%, 85/914). The detection rates of coronavirus, human adenovirus, respiratory syncytial virus A/B, human parainfluenza viruses, human metapneumovirus-A/B, human parechovirus, enterovirus and influenza B virus were 9.9%, 8%, 7.7%, 5%, 3.4%, 3.1%, 3%, and 2.8%, respectively. Conclusions: The most detected viral agents in our study were influenza A virus and rhinovirus. Laboratory diagnosis of respiratory viruses is helpful to prevent unnecessary antibiotic use and is essential in routine diagnostics for antiviral treatment. Multiplex Real-time PCR method is fast and useful for the diagnosis of viral respiratory infections. Copyright
coronavirus , influenza virus , multiplex real-time PCR , Respiratory viruses , rhinovirus
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Department of Medical Microbiology, Faculty of Medicine, Baskent University, Ankara, Turkey
Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey
Faculty of Medicine, Akhmet Yassawi International Kazakh-Turkish University, Turkestan, Kazakhstan
Department of Pediatrics, Faculty of Medicine, Gazi University, Ankara, Turkey
Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey
Division of Medical Virology, Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey
Department of Medical Microbiology
Department of Medical Microbiology
Faculty of Medicine
Department of Pediatrics
Department of Infectious Diseases and Clinical Microbiology
Division of Medical Virology
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