Promising vaccine models against astrovirus MLB2 using integrated vaccinomics and immunoinformatics approaches
Ali S.L. Ali A. Ullah W. Khan A. Khatrawi E.M. Malik A. Abduldayeva A. Baiduissenova A. Althagafi H.J. Fallatah D.
25 September 2024Royal Society of Chemistry
Molecular Systems Design and Engineering
2024#9Issue 121285 - 1299 pp.
Astrovirus MLB2 (AstV-MLB2) is an emerging gastrointestinal virus causing meningitis and disseminated infections. Currently, there are no vaccine-based therapies available for AstV-MLB2. This study aims to develop multi-epitope vaccine models using candidate proteins from AstV-MLB2. Highly immunogenic epitopes (IC50 < 200 μM) exhibiting conservation, antigenicity, and non-allergenicity were called from these proteins. Additionally, the selection criteria for epitopes were based on their potential to trigger immune cells and stimulate IFN-γ-mediated immune responses. The model vaccine constructs were designed from identified lead epitopes, along with immune-enhancer adjuvants and linker sequences. The proposed vaccine models were assessed for allergenicity, antigenicity, and structural integrity to ensure their safety and effectiveness. The binding potential of the vaccine models to HLA and TLR-4 immune cell receptors was evaluated to identify their capacity to stimulate immune responses. Among several raw constructs, MLB2-V1 and MLB2-V2 were identified as potential vaccine candidates due to their non-allergenic features, enhanced antigenic properties, and structural stability. Both these constructs were extensively evaluated and predicted to effectively bind to and interact with immune cell receptors, potentially triggering cellular and innate immune responses. Additionally, the prioritized constructs were deemed suitable for cloning and expression using recombinant DNA systems. The model vaccine constructs showed promise, warranting further investigation into their immune efficacy against MLB2-mediated infections through experimental assays and clinical trials.
Text of the article Перейти на текст статьи
Department of Biochemistry, Abdul Wali Khan University Mardan (AWKUM), Mardan, 23200, Pakistan
Department of Medical Microbiology and Immunology, College of Medicine, Taibah University, Madinah, 42353, Saudi Arabia
Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia
Preventive Medicine, Astana Medical University, Astana, 010000, Kazakhstan
Department of Microbiology and Virology, Astana Medical University, Astana, 010000, Kazakhstan
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Saudi Arabia
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Saudi Arabia
Department of Biochemistry
Department of Medical Microbiology and Immunology
Department of Pharmaceutics
Preventive Medicine
Department of Microbiology and Virology
Department of Biology
Department of Medical Laboratory Sciences
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026