Assessment of the anticancer potential of certain phenolic and flavonoid components in ginger capsules using colorectal cancer cell lines coupled with quantitative analysis
Al Azzam K.M. Al-Areer N.W. Al Omari R.H. Al-Deeb I. Bounoua N. Negim E.-S. Al-Samydai A. Aboalroub A.A. Said R.
October 2024John Wiley and Sons Ltd
Biomedical Chromatography
2024#38Issue 10
Colorectal cancer (CRC) is the fourth most common cause of malignant tumor death. The development of novel, more effective drugs is desperately needed to treat CRC. Zingiber officinale is believed to possess anticancer properties due to its flavonoids and phenols. Using Soxhlet (SOXT) and maceration (MACR) techniques, the present study aimed to evaluate the amounts of quercetin, gallic acid, rutin, naringin, and caffeic acid in ginger capsules of Z. officinale. High-performance liquid chromatography (HPLC)/ultraviolet was used for separation and quantitation. In vitro toxicity evaluation of ginger capsules on the CRC cell line HT-29 was also conducted to assess the anticancer activity of the supplement. The cell line HT-29 (HTB-38) colorectal adenocarcinoma was utilized for the antiproliferative effect of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide. Ginger herbal supplement extract at dosages of 200 and 100 μg had strong cytotoxic effects (IC50 < 50 μg/mL) on HT-29 CRC cells via MACR. This extract is comparable to the SOXT extract, which has an IC50 of less than 50 μg/mL. The anticancer effect of ginger herbal supplement formulations against CRC lines was investigated, and the results obtained from both the MACR and SOXT extraction procedures were noteworthy. The quercetin content was the highest of all the extracts according to the HPLC data.
cancer , ginger capsules , HPLC , HT-29 cells , HTB-38 , Soxhlet , Zingiber officinale
Text of the article Перейти на текст статьи
Department of Chemistry, Faculty of Science, The University of Jordan, Amman, 11942, Jordan
Department of Pharmaceutical Sciences, Pharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman University, Amman, 19328, Jordan
Department of Biopharmaceutics and Clinical Pharmacy, Pharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman University, Amman, 19328, Jordan
Laboratory of the Innovation Sponsorship and the Emerging Institution for Graduates of Higher Education of Sustainable Development and Dealing with Emerging Conditions, Department of Exact Sciences, Normal Higher School of Bechar, Bechar, 8000, Algeria
Laboratory of Chemical and Environmental Science (LCSE), Bechar, 8000, Algeria
School of Petroleum Engineering, Satbayev University, 22 Satpayev Street, Almaty, 050013, Kazakhstan
School of Materials Science and Green Technologies, Kazakh-British Technical University, 59 Tole bi St., Almaty, 050000, Kazakhstan
Department of Chemistry
Department of Pharmaceutical Sciences
Department of Biopharmaceutics and Clinical Pharmacy
Laboratory of the Innovation Sponsorship and the Emerging Institution for Graduates of Higher Education of Sustainable Development and Dealing with Emerging Conditions
Laboratory of Chemical and Environmental Science (LCSE)
School of Petroleum Engineering
School of Materials Science and Green Technologies
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026