Prevalence of Beijing Central Asian/Russian Cluster 94-32 among Multidrug-Resistant M. tuberculosis in Kazakhstan
Akhmetova A. Bismilda V. Chingissova L. Filipenko M. Akilzhanova A. Kozhamkulov U.
January 2024Multidisciplinary Digital Publishing Institute (MDPI)
Antibiotics
2024#13Issue 1
The Beijing genotype is the most distributed M. tuberculosis family in Kazakhstan. In this study, we identified dominant Beijing clusters in Kazakhstan and assessed their drug susceptibility profiles and association with the most widely spread mutation Ser531Leu of the rpoB gene and the mutation Ser315Thr of the katG gene associated with resistance to rifampicin and isoniazid, respectively. M. tuberculosis isolates (n = 540) from new TB cases were included in the study. MIRU-VNTR genotyping was performed for 540 clinical isolates to determine M. tuberculosis families using 24 loci. RD analysis was additionally performed for the Beijing isolates. The identification of mutations in the drug-resistance genes of M. tuberculosis was performed with allele-specific real-time PCR and Sanger sequencing. The Beijing genotype was identified in 60% (324/540) of the clinical isolates. Central Asian/Russian cluster 94-32 was the most distributed cluster among the Beijing isolates (50.3%; 163/324). Three other dominant Beijing clusters were identified as 94-33 (3.4%; 11/324), 100-32 (3.1%; 10/324) and 99-32 (3.1%; 10/324). The Beijing genotype was associated with drug-resistant TB (p < 0.0001), including multidrug-resistant TB (p < 0.0001), in our study. An association of the mutation Ser531Leu of the rpoB gene with the Beijing genotype was found (p < 0.0001; OR = 16.0000; 95%CI: 4.9161–52.0740). Among the Beijing isolates, cluster 94-32 showed an association with MDR-TB (p = 0.021). This is why the evaluation of the Beijing genotype and its clusters is needed to control MDR-TB in Kazakhstan.
Beijing genotype , Central Asian/Russian type 94-32 , MIRU-VNTR , multidrug-resistance , pulmonary tuberculosis
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Laboratory of Genomic and Personalized Medicine, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Astana, 010000, Kazakhstan
Department of General Biology and Genomics, L.N. Gumilyov Eurasian National University, Astana, 010000, Kazakhstan
National Scientific Center of Phthisiopulmonology of the Republic of Kazakhstan, Almaty, 050000, Kazakhstan
Laboratory of Pharmacogenomics, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, 630000, Russian Federation
Faculty of Natural Sciences, Novosibirsk State University, Novosibirsk, 630090, Russian Federation
Laboratory of Genomic and Personalized Medicine
Department of General Biology and Genomics
National Scientific Center of Phthisiopulmonology of the Republic of Kazakhstan
Laboratory of Pharmacogenomics
Faculty of Natural Sciences
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