Hepatoprotective Activity and Oxidative Stress Reduction of an Arctium tomentosum Mill. Root Extract in Mice with Experimentally Induced Hepatotoxicity
Aitynova A. Sevastre B. Ielciu I. Hanganu D. Olah N.-K. Ibragimova N. Shalakhmetova T. Benedec D. Lyu M. Krasnoshtanov A. Gapurkhaeva T.
December 2024Multidisciplinary Digital Publishing Institute (MDPI)
Livers
2024#4Issue 4696 - 710 pp.
Background: The use of natural hepatoprotective remedies represents an important path in modern phytotherapy. Objectives: In this context, our research aims to evaluate the phytochemical composition and the hepatoprotective and oxidative stress reduction potential of an Arctium tomentosum Mill. root extract. Methods: The phenolic profile of the tested extract, prepared by the subcritical fluid-assisted method were qualitatively and quantitatively analyzed by spectrophotometrical and HPLC/DAD/ESI methods. In vitro antioxidant capacity was assessed using DPPH and FRAP assays. Hepatoprotective activity of the extract was assessed on a model of CCl4 experimentally induced hepatotoxicity in mice. Results: Phytochemical assays revealed the presence of important polyphenols, such as chlorogenic acid (17.20 ± 0.65 μg/mL) and acacetin 7-O-glucoside (56.80 ± 1.66 μg/mL). In vitro, the tested extract exhibited a significant oxidative stress reduction capacity, while in vivo it showed a dose-dependent hepatoprotective effect indicated by an improvement in plasma proteins profile and down-regulation of plasma transaminase activity (ALAT, ASAT, GGT). In liver tissue, the extract partially restored the activity of GPx, CAT, and SOD and attenuated lipid peroxidation. The protective effect of the A. tomentosum root extract was supported by the alleviation of histological injuries of the liver (centrilobular necrosis, granulocytic infiltrate, and fibrosis). Conclusions: The A. tomentosum subcritical fluid-assisted root extract proved to be able to provide a significant hepatoprotective effect mainly through an antioxidant mechanism.
antioxidant , Arctium tomentosumMill , hepatoprotective , subcritical fluid-assisted extract
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Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Scientific Center for Anti-Infectious Drugs, Almaty, 050060, Kazakhstan
Department of Clinic and Paraclinic Sciences, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 400372, Romania
Department of Pharmaceutical Botany, Faculty of Pharmacy, Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, 400337, Romania
Department of Pharmacognosy, Faculty of Pharmacy, Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, 400010, Romania
PlantExtrakt Ltd., Rădaia, Cluj-Napoca, 407059, Romania
Department of Medicinal Chemistry and Pharmaceutical Industry, Faculty of Pharmacy, “Vasile Goldiş” Western University of Arad, Arad, 310414, Romania
Faculty of Biology and Biotechnology
Scientific Center for Anti-Infectious Drugs
Department of Clinic and Paraclinic Sciences
Department of Pharmaceutical Botany
Department of Pharmacognosy
PlantExtrakt Ltd.
Department of Medicinal Chemistry and Pharmaceutical Industry
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