Association between the baseline gene expression profile in periapical granuloma and periapical wound healing after surgical endodontic treatment
Ahmed M.A. Nazim F. Ahmed K. Bari M.F. Abdulwahed A. AlMokhatieb A.A. Alalvi Y. Abduljabbar T. Mughal N. Abidi S.H.
December 2022Nature Research
Scientific Reports
2022#12Issue 1
In this study, we have investigated the association between the baseline gene expression profile in periapical granuloma and periapical wound healing after surgical endodontic treatment. Twenty-seven patients aged between 15 and 57 years underwent periapical surgery. The retrieved periapical tissue sample was used for mRNA expression analysis of COL1A1, VTN, ITGA5, IL-4, TNF, ANGPT, VEGFA, and CTGF. All patients were recalled after 6 and 12 months for periapical healing evaluation. Healing was then correlated with baseline gene expression. Healing was observed in 15 patients at the end of 6 months, which increased to 21 patients after 12 months. Six patients showed no healing even after 12 months. Analysis of baseline expression levels of the tested genes with healing status showed the mean relative expression of VTN, VEGFA, ANGPT, TNF, and CTGF to be significantly different (p < 0.05) between the healing group (6 and 12 months) (72.99%) and the non-healing (94.42%) group. Periapical Index scores 3–5 exhibited a positive correlation with ITGA-5 expression. Overexpression of ANGPT and a strong positive correlation between ITGA5 and PAI scores in the non-healing group of patients may suggest these genes to be a potential prognostic biomarker for periapical wound non-healing after surgical endodontic treatment.
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Department of Restorative Dental Sciences, College of Dentistry, King Faisal University, Al-Ahsa, Saudi Arabia
Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan
Department of Pathology, Dr. Ishrat-ul-Ebad Khan Institute of Oral Health Sciences, Dow University of Health Sciences, Karachi, Pakistan
Department of Conservative Dental Sciences, College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
College of Dentistry, King Saud University, Riyadh, Saudi Arabia
Department of Prosthetic Dental Science, College of Dentistry, King Saud University, Riyadh, Saudi Arabia
Department of Surgery, Aga Khan University, Karachi, Pakistan
Department of Biomedical Sciences, Nazarbayev University School of Medicine, Nur-Sultan, Kazakhstan
Department of Restorative Dental Sciences
Department of Biological and Biomedical Sciences
Department of Pathology
Department of Conservative Dental Sciences
College of Dentistry
Department of Prosthetic Dental Science
Department of Surgery
Department of Biomedical Sciences
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