Tissue Extract from Brittle Star Undergoing Arm Regeneration Promotes Wound Healing in Rat
Afshar A. Khoradmehr A. Nowzari F. Baghban N. Zare M. Najafi M. Keshavarzi S.Z. Zendehboudi F. Mohebbi G. Barmak A. Mohajer F. Basouli N. Keshtkar M. Iraji A. Sari Aslani F. Irajie C. Nabipour I. Mahmudpour M. Tanideh N. Tamadon A.
July 2023Multidisciplinary Digital Publishing Institute (MDPI)
Marine Drugs
2023#21Issue 7
This study set out to evaluate the wound healing properties of brittle star extracts in vitro and in vivo. Due to the great arm regeneration potential of the brittle star, Ophiocoma cynthiae, the present study aimed to evaluate the wound healing effect of hydroalcoholic extracts of brittle star undergoing arm regeneration in wound healing models. The brittle star samples were collected from Nayband Bay, Bushehr, Iran. After wound induction in the arm of brittle stars, hydroalcoholic extracts relating to different times of arm regeneration were prepared. The GC-MS analysis, in vitro MTT cell viability and cell migration, Western blot, and computational analysis tests were performed. Based on the in vitro findings, two BSEs were chosen for in vivo testing. Macroscopic, histopathological and biochemical evaluations were performed after treatments. The results showed positive proliferative effects of BSEs. Specifically, forty-two compounds were detected in all groups of BSEs using GC-MS analysis, and their biological activities were assessed. The MTT assay showed that the 14 d BSE had a higher proliferative effect on HFF cells than 7 d BSE. The cell migration assay showed that the wound area in 7 d and 14 d BSEs was significantly lower than in the control group. Western blot analysis demonstrated an increase in the expression of proliferation-related proteins. Upon the computational analysis, a strong affinity of some compounds with proteins was observed. The in vivo analysis showed that the evaluation of wound changes and the percentage of wound healing in cell migration assay in the 7 d BSE group was better than in the other groups. Histopathological scores of the 7 d BSE and 14 d BSE groups were significantly higher than in the other groups. In conclusion, the hydroalcoholic extract of O. cynthiae undergoing arm regeneration after 7 and 14 days promoted the wound healing process in the cell and rat skin wound healing model due to their proliferative and migratory biological activity.
brittle star , proliferation , rat , regeneration , wound healing
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PerciaVista R&D Co, Shiraz 73, Iran
Student Research Committee, Bushehr University of Medical Sciences, Bushehr 75, Iran
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 75, Iran
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz 73, Iran
Food Lab, Bushehr University of Medical Sciences, Bushehr 75, Iran
Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz 73, Iran
Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz 73, Iran
Molecular Dermatology Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz 73, Iran
Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz 73, Iran
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr 75, Iran
Department of Pharmacology, Medical School, Shiraz University of Medical Sciences, Shiraz 73, Iran
Department for Scientific Work, Ospanov Medical University, West Kazakhstan Marat, Aktobe, 030012, Kazakhstan
PerciaVista R&D Co
Student Research Committee
The Persian Gulf Marine Biotechnology Research Center
Stem Cells Technology Research Center
Food Lab
Medicinal and Natural Products Chemistry Research Center
Central Research Laboratory
Molecular Dermatology Research Center
Department of Medical Biotechnology
The Persian Gulf Tropical Medicine Research Center
Department of Pharmacology
Department for Scientific Work
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