Electrofermentation increases concentration of poly γ-glutamic acid in Bacillus subtilis biofilms
Adilkhanova A. Ormantayeva A. Kaziullayeva A. Olaifa K. Eghtesadi N. Abbas A.H. Calvio C. Pham T.T. Ajunwa O.M. Marsili E.
March 2024John Wiley and Sons Ltd
Microbial Biotechnology
2024#17Issue 3
Fluctuations in redox conditions in bioprocesses can alter the end-products, reduce their concentration, and lengthen the process time. Electrofermentation enables rapid metabolic modulation of biosynthesis and allows control of redox imbalances in biofilm-based fermentation processes. In this study, electrofermentation is used to boost the production of the bacterial biopolymer poly-γ-glutamic acid (γ-PGA) from Bacillus subtilis ATCC 6051. When compared to control experiments (3.3 ± 0.99 g L−1), the application of an electrode potential E = 0.4 V versus Ag/AgCl results in a more than two-fold increase in the production of γ-PGA (9.13 ± 1.4 g L−1). Using an engineered B. subtilis strain, in which γ-PGA production is driven by isopropyl β-d-1-thiogalactopyranoside, electrofermentation improves polymer concentrations from 15.4 ± 1.5 to 23.1 ± 1.6 versus g L−1. These results confirm that electrofermentation conditions can be adopted to increase the concentration of γ-PGA and perhaps other extracellular biopolymers in industrial strains.
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Biofilm Laboratory, Department of Chemical and Materials Engineering, School of Engineering and Digital Sciences, Nazarbayev University, Astana, Kazakhstan
Department of Petroleum Engineering, School of Mining and Geosciences, Nazarbayev University, Astana, Kazakhstan
Nottingham Ningbo China Beacons of Excellence Research and Innovation Institute, Ningbo, China
Department of Biology and Biotechnology, Università degli Studi di Pavia, Pavia, Italy
Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan
Department of Biology, Faculty of Natural Sciences, Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark
Biofilm Laboratory
Department of Petroleum Engineering
Nottingham Ningbo China Beacons of Excellence Research and Innovation Institute
Department of Biology and Biotechnology
Department of Biology
Department of Biology
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