Isolation of Bacillus thuringiensis Strains to Create a Biological Pesticide for Agricultural Development and Environmental Sustainability
Adilkhankyzy A. Tleubergenov K.M. Shissenbayeva N.Z. Uspanov A.M. Duisembekov B.A.
Jan-Feb 2025Unique Scientific Publishers
International Journal of Agriculture and Biosciences
2025#14Issue 1100 - 108 pp.
This study aimed to isolate and identify highly virulent Bacillus thuringiensis (Bt) strains to develop biopesticides. Extensive field studies were conducted in nature reserves and woodlands to collect samples from soils and dead insects showing symptoms of bacterial infection. A total of 530 samples were collected, from which 30 Bt isolates were obtained and subjected to detailed physiological and biochemical analysis. The isolates were identified serologically and divided into three subspecies: Bt kurstaki, Bt sotto, and Bt toguchini. The biological efficiency of these strains was evaluated against eight species of lepidoptera pests in controlled laboratory conditions. Strains k-Ym07/CB and 2127-3k demonstrated the highest biological activity, reaching 100% mortality of the target insect species within five days. The results show that Bt kurstaki strains k-Ym07/CB and 2127-3k have significant potential as biological pesticides for controlling lepidoptera pests in Kazakhstan. The study concludes that developing these biological pesticides can offer an environmentally sustainable alternative to chemical pesticides, increasing agricultural productivity while maintaining an ecological balance.
Bacillus thuringiensis strains , Biological efficiency , Sustainable ecology
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Kazakh Research Institute of Plant Protection and Quarantine, named after Zhazken Zhyembayev, Almaty, Kazakhstan
Kazakh Research Institute of Plant Protection and Quarantine
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