Genotypes of the Congo-Crimean Hemorrhagic Fever Virus Occurring in the Turkestan Region
Abuova G.N. Pshenichnaya N. Karan’ L.S. Berdaliyeva F.A. Aliyev D.S. Sadyhova D.K. Polukchi T.V. Nurmagambet S.D.
1 December 2022Brieflands
Archives of Clinical Infectious Diseases
2022#17Issue 6
Background: The Turkestan region of Kazakhstan is the natural focus of the Congo-Crimean hemorrhagic fever (CCHF). Every year, some of the patients treated in hospitals are not included in official statistics and remain in the group of possible cases of CCHF. Objectives: This study aimed to verify the genotypes of the CCHF virus (CCHFV) in the Turkestan region and Shymkent City. Methods: Twelve blood samples from patients with CCHF were studied, the diagnosis of which was verified on the basis of posi-tive specific immunoglobulins IgM to the virus. To isolate viral RNA, we used a special MAGNO-sorb kit. To detect RNA, CCHFV was used with a set of Amplisens CCHFV-FL by real-time polymerase chain reaction (PCR) using a Rotor-Gene Q device. For the reverse transcription reaction, we used a set of Reverta-L. Results: The study of the genome sequence of CHFV from GenBank demonstrated that isolates from the Turkestan region belonged to genetic groups Asia-1 and Asia-2. Conclusions: For the first time in Kazakhstan’s history, a phylogenetic analysis of RNA sequences of viruses from patients with CCHF was performed in the Turkestan region, as a result of which genetic groups Asia-1, reassortant Asia-1, and Asia-2 were established.
Congo-Crimean Hemorrhagic Fever , Genotypes , Kazakhstan , Tick-borne Illness , Virus
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South Kazakhstan Medical Academy, Shymkent, Kazakhstan
Central Research Institute of Epidemiology, Moscow, Russian Federation
South Kazakhstan Medical Academy
Central Research Institute of Epidemiology
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