Phytochemical and Micro-Morphological Characterization of Atraphaxis pyrifolia Bunge Growing in the Republic of Kazakhstan
Abilkassymova A. Kozykeyeva R. Aldana-Mejía J.A. John Adams S. Datkhayev U. Turgumbayeva A. Orynbassarova K. Saroja S.G. Khan I.A. Ross S.A.
February 2024Multidisciplinary Digital Publishing Institute (MDPI)
Molecules
2024#29Issue 4
Atraphaxis pyrifolia is a native species of Central Asia, known for curing several disorders. The species has little knowledges about its chemical composition and any information about its morphological characteristics despite its importance in traditional Asian medicine. This is one of the first approaches to the phytochemical and morphological characterization of this species. Micro-morphology was performed on the stem, and leaf parts of this plant to profile the morpho-anatomical characters using brightfield, fluorescence, polarized and scanning electron microscopy. Leaves were extracted with hexane and methanol. The hexane extract was analyzed using GC-MS analysis revealing the major presence of γ-sitosterol and nonacosane. The methanolic extract was submitted to Vacuum Liquid Chromatography and Sephadex LH-20. HPTLC, HR-ESI-MS and NMR techniques were used to identify the main compounds. Four glycosylated flavonoids were isolated: 8-O-acetyl-7-O-methyl-3-O-α-l-rhamnopyranosylgossypetin (Compound 1), and 7-O-methyl-3-O-α-l-rhamnopyranosylgossypetin (Compound 3), and two other compounds reported for the first time in the literature (Compounds 2 and 4). The findings presented herein furnish pertinent information essential for the identification and authentication of this medicinal plant. Such insights are invaluable for facilitating robust quality control measures and serve as a foundational framework for subsequent endeavours in metabolic, pharmacological, and taxonomical analyses.
Atraphaxis pyrifolia , flavonoids , HPTLC , micro-morphology , triterpenes
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Higher School of Medicine, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
School of Pharmacy, Asfendiyarov Kazakh National Medical University, Almaty, 050012, Kazakhstan
National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, Oxford, 38677, MS, United States
Department of Pharmacognosy, Faculty of Pharmacy, South Kazakhstan Medical Academy, Shymkent, 160019, Kazakhstan
School of Life Sciences, University of Westminster, London, W1W 6UW, United Kingdom
Department of Biomolecular Sciences, Division of Pharmacognosy, School of Pharmacy, University of Mississippi, Oxford, 38677, MS, United States
Higher School of Medicine
School of Pharmacy
National Center for Natural Products Research
Department of Pharmacognosy
School of Life Sciences
Department of Biomolecular Sciences
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