Umbilical cord blood immune cell composition in preterm infants with and without bronchopulmonary dysplasia: A pilot CIBERSORT analysis
Бронхөкпелік дисплазиясы бар және онсыз шала туған нәрестелердің кіндік қанының жасушалық құрамын CIBERSORT әдісімен пилоттық зерттеу
Пилотное исследование клеточного состава пуповинной крови у недоношенных детей с бронхолегочной дисплазией и без нее на основе метода CIBERSORT
Abilbayeva A. Tarabayeva A. Isgandarov I. Okhas I. Shaktay N.
30 December 2025Kaz Med Print LLP
Reproductive Medicine (Central Asia)
2025#2025Issue 4243 - 250 pp.
Relevance: Bronchopulmonary dysplasia (BPD) remains a common chronic disease of very low birth weight preterm infants, and despite the known role of chronic inflammation and immune imbalance in its pathogenesis, the precise mechanisms and early predictors remain poorly understood. This study aimed to identify early cellular markers of bronchopulmonary dysplasia in very low birth weight neonates. Materials and Methods: Cell-type Identification by Estimating Relative Subsets of RNA Transcripts (CIBERSORT) enables immune cell profiling by deconvolving gene expression microarray data. Series GSE220135, including 13 cord blood samples from individuals with BPD and 55 without BPD, was obtained from the Gene Expression Omnibus (GEO) database. Results: CIBERSORT deconvolution revealed significantly elevated eosinophil levels (p = 0.010) and reduced M2 macrophage levels (p = 0.024) in the cord blood of neonates who developed BPD. Correlation analysis revealed a strong positive association between M1 macrophages and activated mast cells (r = 0.60, p = 0.008) and a strong negative correlation between neutrophils and CD8 cytotoxic T lymphocytes (r = -0.62, p < 0.001). Eosinophil levels exhibited moderate negative correlations with gestational age (r = -0.45, p = 0.021) and birth weight (r = -0.47, p = 0.015), respectively, whereas M2 macrophages showed no significant correlation with these parameters. Conclusion: The presented results of a pilot CIBERSORT analysis of cord blood suggest a link between the composition of cell fractions and the subsequent development of bronchopulmonary dysplasia. The statistically significant increase in the eosinophil fraction and decrease in the M2 macrophage fraction in the cord blood of preterm infants with BPD may indicate an association of these cells with an intrauterine inflammatory phenotype predisposing to the development of BPD. Â
bronchopulmonary dysplasia (BPD) , CIBERSORT , innate immunity , very-low-birth-weight infant
Text of the article Перейти на текст статьи
Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan
Asfendiyarov Kazakh National Medical University
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026