Identification of significant genes and signaling pathways in bronchopulmonary dysplasia in preterm newborns using bioinformatics analysis

Идентификация значимых генов и сигнальных путей при бронхолегочной дисплазии у недоношенных новорожденных с помощью биоинформационного анализа
Abilbayeva A.A. Tarabayeva A.S. Isgandarov I.V. Smagul A.O.
2025 Dynasty Publishing House

Voprosy Ginekologii, Akusherstva i Perinatologii
2025 #24 Issue 4 26 - 35 pp.

Objective. To identify potentially significant genes and key pathways involved in the pathogenesis of bronchopulmonary dysplasia (BPD) in preterm newborns using bioinformatics analysis. Materials and methods. The GSE219156 data from GEO was used for bioinformatics analysis. DEG identification was performed using the GEO2R web tool. GO and KEGG functional analysis was performed using the ClusterProfiler package. Reactome analysis was performed using the Enrichr tool. The results were visualized using the SRplot platform. The PPI network for differentially expressed genes (DEGs) was constructed using the STRING website and visualized in Cytoscape. The cytoHubba plugin was used to identify hub genes. The Mann-Whitney U test and t-test were used to compare groups. Results. Of the 137 identified DEGs, 47 showed low expression and 90 showed elevated expression. Ten DEGs were identified as hub genes, including CD27, IFNG, KLRK1, KLRD1, TBX21, EOMES, CXCR6, NCR1, CD19, and CCL5. Among them, IFNG and KLRK1 were key participants in protein-protein interactions (PPI). At the same time, humoral adaptive immunity showed its significance as a biological process, while antigen processing and presentation and natural killer cell-mediated cytotoxicity were identified as key signaling pathways. Conclusion. The results of this study indicate that IFNG and KLRK1 can be considered as potentially significant genes in the pathogenesis of BPD.

bioinformatics analysis , bronchopulmonary dysplasia , gene expression , hub genes

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A.A.Shortanbayev Department of General Immunology, Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan
Department of Natural Sciences, SDU University, Kaskelen, Kazakhstan
International Faculty, Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan

A.A.Shortanbayev Department of General Immunology
Department of Natural Sciences
International Faculty

10 лет помогаем публиковать статьи Международный издатель

Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026