Comparative Antitumor and Mechanistic Effects of the Iodine Lithium Complex KC-144 in Cisplatin-Sensitive, Cisplatin Resistant and Non-Malignant Mesothelial Cells

Abekova A. Kenzhebekova R. Jumagaziyeva A. Turganbay S. Ilin A. Ossikbayeva S. Kydyrbekova A. Islamov R.
February 2026 Engineered Science Publisher

Engineered Science
2026 #39

Pleural mesothelioma (PM) is an aggressive cancer with limited treatment options, particularly in cisplatin-resistant cases. This study evaluated the anticancer activity of a novel iodine-coordinated compound, dilithium (I) (1→4)-L-D-polyglucopyran triiodide chloride, polypeptide “KC-144,” in cisplatin-sensitive and cisplatin-resistant mesothelioma cell models. Cytotoxic and antiproliferative effects were assessed using MTT assays, colony-forming assays, wound-healing tests, cell cycle analysis, apoptosis detection, mitochondrial membrane potential measurements, and quantification of reactive oxygen and nitrogen species. KC-144 exhibited IC₅₀ values of 0.416 ± 0.046 mg mL⁻¹ in P31 cells, 0.399 ± 0.014 mg mL⁻¹ in cisplatin-resistant P31res1.2 cells, and 0.591 ± 0.021 mg mL⁻¹ in non-malignant MeT-5A cells, indicating moderate selectivity toward malignant cells. Treatment induced oxidative stress, mitochondrial dysfunction, apoptosis, and G₀/G₁ cell-cycle arrest in mesothelioma cells, accompanied by concentration-dependent inhibition of clonogenic growth. These findings suggest that KC-144 triggers apoptotic pathways at moderate concentrations and direct cytotoxic effects at higher doses. Although the results demonstrate promising antitumor activity, the study is limited by its in vitro design and the narrow therapeutic window observed in normal cells, warranting further mechanistic investigations and in vivo validation before clinical translation.

Apoptosis , Cell cycle , Cisplatin , Iodine , Pleural mesothelioma , Reactive oxygen species

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Department of Molecular Biology and Genetics, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, 71 Al-Farabi Avenue, Almaty, 050040, Kazakhstan
Research and Production Enterprise Antigen LTD, Karasai district, Abay village, 4 Azerbayev Street, Almaty, 040905, Kazakhstan
JSC «Scientific Center for Anti-Infectious Drugs», 75A Al-Farabi Avenue, Almaty, 050060, Kazakhstan
JS «KazIOR» Molecular Genetic Research Center, 91 Abay, Almaty, 050040, Kazakhstan
Information and technical department, College of Management, 14/3 Orlykol street, Astana, 010000, Kazakhstan
Department of Chemistry and Technology of Organic Substances, Natural Compounds and Polymers, Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, 71 Al-Farabi Avenue, Almaty, 050040, Kazakhstan

Department of Molecular Biology and Genetics
Research and Production Enterprise Antigen LTD
JSC «Scientific Center for Anti-Infectious Drugs»
JS «KazIOR» Molecular Genetic Research Center
Information and technical department
Department of Chemistry and Technology of Organic Substances

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